Alejandro Martin

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Questions related from Alejandro Martin

Is the E. coli MACH1 strain resistant to hygromycin?

We are trying to transform a HygR construct into MACH1, using Hyg at 100 ug/mL as selection. However we keep getting a bacterial lawn even in the non-transformed control, using two different...

08 August 2019 3,605 13 View

In vivo biotinylation of T7 promoter-driven constructs?

I am thinking of buying a ColE1-compatible plasmid to co-express biotin ligase (BirA) in E. coli, with the intention of biotinylating in vivo our Avi-tagged constructs. Whatever we end up buying...

01 March 2018 3,691 2 View

Accumulation of pyrophosphate during PCR?

I recently came across this article: Pyrophosphate hydrolysis is an intrinsic and critical step o... According to which the amount of pyrophosphate accumulated during a PCR reaction (or any other...

01 January 1970 2,821 4 View