I am looking for a direct method to quantify Spirulina biomass. What kind of slide should I use to count cells or filaments?
My school recently acquired a Veeco Multimode and Nanoscope IIIa. While our software and computer is set up and working, the actual Nanoscope is not powering on. We currently have it wired up, and...
19 June 2024 592 0 View
Hydrogen is seen a promising decarbonisation route for many energy-related sectors. However, like for other energy innovations, the NIMBY syndrome may turn it into something socially...
30 May 2024 7,063 0 View
The professor gave us an exercise but for me it's not totally clear. I have a distribution function which depend on a parameter X that I need to estimate. The function describes the distribution...
03 January 2024 603 0 View
Dear friends, I want to obtain a graph by doing Principle Response Curve analysis in R. I can reach the result using the sample code with the 'vegan' package and 'pyrifos' data. However, I cannot...
01 January 2024 5,081 0 View
I am trying to prepare Llyod WPM medium but it did not solidify and remained in gel form. I was only add 2.1 g of agar and 0.72 g of Llyod for 300 ml. What should I do?
28 September 2023 3,853 0 View
I exposed some organisms to BPA at low concentrations (10-100 µg/L) for 1 month and now I need to get the bekers clean. Does anybody know if and how much BPA sticks to glass and which is the best...
27 July 2023 3,185 0 View
It says they are updating the developer software, but has been like this for a week. Any updates on this? I would like to gather data from this platform.
30 May 2023 3,810 1 View
Hello, I am new in RNA FISH and I want to use Qiagen LNA probes that I designed through GeneGlobe tool of the company. The probes are 20-22 in size and the site gives no chance to increase. Is...
05 March 2023 6,827 0 View
I need layers for spatial analysis of Sakarya province in the eastern Marmara region of Turkey. Where can I find it for free? Thank you in advance!
14 February 2023 6,401 9 View
To purify the protein, I am using 0.5% Triton X-100 because it is hydrophobic. Lysis buffer has ne contain imidazole and elution buffer has 250 mM imidazole. I tried to optimize imidazole...
17 August 2022 9,456 8 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,822 3 View
I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...
08 August 2024 4,810 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,104 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 639 2 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,850 1 View