I am trying to prepare Llyod WPM medium but it did not solidify and remained in gel form. I was only add 2.1 g of agar and 0.72 g of Llyod for 300 ml. What should I do?
Which ELISA kit brand would you recommend for rat TDP-43 (25, 35, and 45 kDa) and pTDP-43 (Ser 409/410) tissue homogenates?
29 February 2024 8,568 1 View
I'm a teacher of EFL (English as a foreign language) in Turkey. I have used a new teaching model in my face to face classes this semester, and now I'd like to see how much it has affected my...
15 January 2024 4,239 9 View
Dear friends, I want to obtain a graph by doing Principle Response Curve analysis in R. I can reach the result using the sample code with the 'vegan' package and 'pyrifos' data. However, I cannot...
01 January 2024 5,081 0 View
I'm thinking of starting an article about gastronomy tourism soon. For this reason, I need a teammate who can offer different perspectives and produce quality publications and is suitable for...
04 December 2023 8,402 6 View
Based on the Rt-PCR analysis results of the oligos I have designed, it appears that there is a consistent signal from the NTC and negative patient samples with Ct values ranging from 30 to 32....
19 June 2023 3,010 6 View
Hello, I am new in RNA FISH and I want to use Qiagen LNA probes that I designed through GeneGlobe tool of the company. The probes are 20-22 in size and the site gives no chance to increase. Is...
05 March 2023 6,827 0 View
I need layers for spatial analysis of Sakarya province in the eastern Marmara region of Turkey. Where can I find it for free? Thank you in advance!
14 February 2023 6,401 9 View
I am looking for a direct method to quantify Spirulina biomass. What kind of slide should I use to count cells or filaments?
29 January 2023 9,079 0 View
Hi. Is there a program, app, website or software that can automatically guide people through the steps of the research methodology they should pursue by simply asking them certain questions about...
20 November 2022 9,767 3 View
Hi. Consider this: I gave a treatment to my Experiment group (K-12 students) to see if it will affect their motivation while my Control group did not receive the said treatment. I checked the...
19 November 2022 6,006 21 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,478 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,129 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,404 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 949 4 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,662 6 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...
21 July 2024 5,128 4 View