Why did balb/c mice lost their hair one month after subcutaneusly injection of Hela cancer cell?

More Rozita Nasiri's questions See All

How to convert a methyl group (-CH3) to a carboxyl or amine group (-COOH/NH2)?

Which mechanism(s) or linker are needed to change a methyl group (-CH3) to a carboxyl or amine groups (-COOH/_NH2)?

01 February 2019 8,092 10 View

Looking for reviewers for paper in nanotechnology, nano-medicine,nano drug delivery, nano bio-materials, nano ceramics, and nano related fields????

I am looking for reviewers for my paper submission. Could you please advice me what I am suppose to do? Thank you in advance.

09 October 2015 355 2 View

How can I induce immune suppression in normal laboratory balb/c mice using drugs or other simple methods?

Does anybody know that how I can induce immune suppression in balb/c mice using drugs or any other simple method? Needless to say I cannot use nude or SCID mice in our conventional animal lab. On...

08 September 2015 2,893 9 View

Does anybody know about cell line injections in immunocompetent balb/c mice?

I am developing two different cancer models in immunocompetent balb/c mice by subcutaneusly injection of Hela and SK-BR-3 cell lines. Appreciate any suggestions.Is there any option to generate...

07 August 2015 5,653 10 View

What is the right injection amount for SkBr3 cells in white ICR mice?

Is one time injection of cells enough to establish cancer in mice? is 1* 10^7 cells enough or not ? How detect cancer after injection and how determine that is our interested cancer which...

04 May 2015 367 8 View

Can someone help me with the methods of HPLC using C18 column to determine bromelain enzyme concentration in juice?

I am looking for the method to determine bromelain enzyme concentration in unknown sample using HPLC C18 column. I would be very thankful and appreciative if anyone can help me with method and...

31 December 2014 6,822 5 View

Which type of functional group will appear in FTIR analysis as peaks after covalent conjugation of enzyme?

The ―COOH of nanoparticles was activated using EDC/NHS and then covalently attached to the ―NH2 of the enzyme, forming ―CO―NH―. Now, which peaks number will show that the conjugation successfully...

11 December 2014 5,850 9 View

What could be the possible reason for a decreasing XRD intensity after citric acid coating?

Iron oxide nanoparticles are coated with citric acid and the XRD patterns analysed before and after coating with citric acid. The only difference was in intensity which decrease after coating....

10 November 2014 5,163 8 View

How can we tell from an M-H curve that the sample is superparamagnetic?

What is the range of saturation magnetization of superparamagnetic nanoparticles? Why sometimes saturation magnetization come down to 1emu, but sometimes is higher than 60 emu. What is the...

10 November 2014 969 19 View

Does anyone have experience with VSM analysis?

Herewith, the VSM result for synthesized nanoparticles is attached. Does anyone know how to analyse the graf step by step? What could be concluded?

10 November 2014 9,972 15 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Goodwin G. Jinesh

Interesting...!! Think about T-cell defects..!!

Gary Leslie Wright

Get back to me when you stumble across a way to make hair grow back rather than fall out:)

Diğdem YÖYEN ERMİŞ

I think there is a really strong immune respons. If you injected a lots of cells maybe they induced the immune reaction.

Prijovic Mirko Zeljko

Stress itself may cause hair loss at mice. Contamination with hair follicle mites is another reason. May not be related to cells injected.