Is one time injection of cells enough to establish cancer in mice? is 1* 10^7 cells enough or not ? How detect cancer after injection and how determine that is our interested cancer which established in mice?
Hi Rozita,
A few points: Usually 1-5x10^6 breast cancer cells are typically injected either in the mammary fat pad or subcutaneously in immunocompremised mice (nude, athymic, NOD/SCID, NSG, but not ICR!). Once a tumor forms, you will see a bump growing and since these mice don't develop spontaneous tumors subcutaneously or in the fat pad, this is for sure your cancer.
1 x 107 is a very high number of cells for tumor formation. As Richard mentioned, 1-5 x 106 cells are generally sufficient for formation of tumors either subcutaneously or into the mammary fat pads of either NUDE or NOD/SCID mice only. Subcutaneous or mammary fat pad tumors can be easily detected as they start forming a bulge in 10-15 days as they grow.
If you plan to inject SKBr3 cells intravenously into the lungs, you can implant upto 1.5 x 106 cells at the maximum. Tumor formation in this case can be predicted by the weight loss of mice.
It means that is not necessary to do the histological study after tumor formation and we can proceed the experiment. Thanks a lot for the helpful comments. I am going to use BALB/c mice.
It means that is not necessary to do the histological study after tumor formation and we can proceed the experiment. Thanks a lot for the helpful comments. I am going to use BALB/c mice.
Hi Nazita,
SK-BR-3 cell line is a human breast adenocarcinoma. You can't use Balb/c mice for inoculation. You should use Balb/c nude mice or B17.Scid or NOG scid or NSG scid mice or ICR scid mice.
Yes, it is not necessary to do the histological study after tumor formation.
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