Diğdem YÖYEN ERMİŞ

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Questions related from Diğdem YÖYEN ERMİŞ

Could you suggest me any Protocol working with dream taq buffer and polymerase?

I started work with Dream taq polymerase and its buffer but I have primer dimers. For solving this problem I tried gradient PCR from 60 to 66 °C. Is this problem from my primers or my agent?...

02 February 2016 3,155 6 View

Can you suggest me intracellular p-STAT3 staining for peripheral blood monocytes or granulocytes ?

I am working with peripheral blood MDSC and I try to show their p-STAT3 level using with ıntracelluler flow cytometry staining. But there is a specificity problem. Iso and p-STAT3 antibody stained...

01 January 2016 4,195 4 View

Does anyone use flow cytometry for nitric oxide (NO) detection ?

I want to detect nitric oxide (NO) with flow cytometry. I found two agent which are 4,5-Diaminofluorescein diacetate solution and 5,6-Diaminofluorescein diacetate- Sigma-Aldrich. Which is the...

10 October 2015 8,771 9 View

Can anyone recommend some breast cancer cell lines derived from Balb/c or B6?

I need breast cancer cell lines for use them in Balb/c or B6 strains.

05 May 2015 5,576 8 View

Is there anyone working with EL4 cell line?

I injected 500000 cells per animal subcutaneously but I couldn't palpate the Tumor. We are at 17th Day. What is your experience about this type of tumor? How many days should I wait for get tumor?

03 March 2015 8,823 3 View

What are the surface marker differences between young and old neutrophils obtained from mouse ?

How can we separate young and old mouse neutrophils? What are these cell's significant characteristic? What kind of techniques do you use to separate these cells?

03 March 2015 6,071 5 View

Can anyone give me any suggestions for fixing this western problem?

Here is our western results. We try to understand these band appear like this. Do you have any opinion for solving this problem?

02 February 2015 476 4 View

Is there a specific intracellular staining protocol for phosphorylated protein detection?

I am studying with phospho protein and ı want to detect it with flow cytometry using intracellular staining protocol. Do ı need any buffer for protect phospho proteins ?

02 February 2015 6,643 11 View

Transfection of THP-1 cells?

I am trying to transfect THP-1 cell line using with lipofectamine. But it does not work. I want to try lipofectamine before electroporation. Can you give me your protocol ? If the lipofectamine...

01 January 1970 9,129 4 View

Detection of small peptide molecules derived from immune proteins?

I am working with small peptide molecules derived from immune proteins. My previous experiments were about their function. For this reason, I synthesized them using their amino asid sequence....

01 January 1970 5,698 3 View