Rozita Nasiri

21 Questions 46 Answers 0 Followers

Questions related from Rozita Nasiri

How to convert a methyl group (-CH3) to a carboxyl or amine group (-COOH/NH2)?

Which mechanism(s) or linker are needed to change a methyl group (-CH3) to a carboxyl or amine groups (-COOH/_NH2)?

02 February 2019 8,026 10 View

Looking for reviewers for paper in nanotechnology, nano-medicine,nano drug delivery, nano bio-materials, nano ceramics, and nano related fields????

I am looking for reviewers for my paper submission. Could you please advice me what I am suppose to do? Thank you in advance.

10 October 2015 317 2 View

How can I induce immune suppression in normal laboratory balb/c mice using drugs or other simple methods?

Does anybody know that how I can induce immune suppression in balb/c mice using drugs or any other simple method? Needless to say I cannot use nude or SCID mice in our conventional animal lab. On...

09 September 2015 2,852 9 View

Why did balb/c mice lost their hair one month after subcutaneusly injection of Hela cancer cell?

The immunocompetent balb/c mice were injected by Hela cell lines. They lost their hair one month after injection, but there is no cancer bumping yet.

08 August 2015 9,029 4 View

Does anybody know about cell line injections in immunocompetent balb/c mice?

I am developing two different cancer models in immunocompetent balb/c mice by subcutaneusly injection of Hela and SK-BR-3 cell lines. Appreciate any suggestions.Is there any option to generate...

08 August 2015 5,598 10 View

What is the right injection amount for SkBr3 cells in white ICR mice?

Is one time injection of cells enough to establish cancer in mice? is 1* 10^7 cells enough or not ? How detect cancer after injection and how determine that is our interested cancer which...

05 May 2015 313 8 View

Can someone help me with the methods of HPLC using C18 column to determine bromelain enzyme concentration in juice?

I am looking for the method to determine bromelain enzyme concentration in unknown sample using HPLC C18 column. I would be very thankful and appreciative if anyone can help me with method and...

01 January 2015 6,789 5 View

Which type of functional group will appear in FTIR analysis as peaks after covalent conjugation of enzyme?

The ―COOH of nanoparticles was activated using EDC/NHS and then covalently attached to the ―NH2 of the enzyme, forming ―CO―NH―. Now, which peaks number will show that the conjugation successfully...

12 December 2014 5,812 9 View

What is the PEG 5000 melting point?

Iron oxide nanoparticles are coated with PEG 5000 in liquid form and I need to make it powder for further study (such as XRD). As I do not want to change the structure of NPs, I need to find out...

11 November 2014 6,371 15 View

What could be the possible reason for a decreasing XRD intensity after citric acid coating?

Iron oxide nanoparticles are coated with citric acid and the XRD patterns analysed before and after coating with citric acid. The only difference was in intensity which decrease after coating....

11 November 2014 5,108 8 View

Does anyone have experience with VSM analysis?

Herewith, the VSM result for synthesized nanoparticles is attached. Does anyone know how to analyse the graf step by step? What could be concluded?

11 November 2014 9,930 15 View

How can we tell from an M-H curve that the sample is superparamagnetic?

What is the range of saturation magnetization of superparamagnetic nanoparticles? Why sometimes saturation magnetization come down to 1emu, but sometimes is higher than 60 emu. What is the...

11 November 2014 866 19 View

Does anyone have experience with FTIR analysis?

Iron oxide nanoparticles are coated with citric acid and the asymmetric and symmetric –COO- stretching frequencies of CA shifted to higher wavenumbers. Can I conclude that indicates outer sphere...

07 July 2014 8,050 12 View

Can anyone help me with an easy method to determine the proteolytic activity of an enzyme?

proteolytic enzyme is conjugated to drug delivery system and I am going to check whether is still active after conjugation or not.

06 June 2014 4,920 4 View

What is the procedure for determining carboxyl groups by the acid-base titration method in solution?

Iron oxide nanoparticles are coated with modified dextran with COOH group. What is the procedure for determining COOH groups by the acid-base titration method in solution?

01 January 2014 8,156 3 View

How to detect radio-labeled molecules in a solution?

I coated iron oxide nanoparticles with dextran to use as a drug delivery vehicle. Radiolabeled bombesin peptide is conjugated to dextran in the surface of the nanoparticle in order to detect...

12 December 2013 756 7 View

How to quantitatively and qualitatively determine the presence and concentration of two different proteins in one solution?

I coated an iron oxide nanoparticle as a drug delivery vehicle. Bombesin peptide as a targeting agent and bromelain protein nature anticancer agent are conjugated to nanoparticle. I want to detect...

12 December 2013 1,731 6 View

Which type of dialysis bag (14,000 cut-off) is better?

I am going to coat iron oxide nanoparticles with dextran T40 and in the last step the excess ammonia and its leftovers, iron cations and dextran macromolecules should be removed by dialysis using...

11 November 2013 644 22 View

How to conjugate iron oxide nanoparticles with an OH group on its surface to one enzyme and one peptide?

I have iron oxide nanoparticles which have a OH group on its surface and I am going to conjugate them to one enzyme and one peptide at the same time using EDC-sulpho-NHS chemistry: can anyone...

10 October 2013 689 10 View

How does one determine protein concentration in one solution?

I conjugate one protein and one peptide to the polymer iron oxide coated nanoparticle and I am going to determine the protein concentration in my solution, but I do not know how to determine their...

10 October 2013 7,293 11 View

Could someone share the manual protocol for doxorubicin and bromelain loading in peglated iron oxide nanoparticle?

Drug delivery system- I am asking about chemical reactions that conjugate the PEG to DOX and bromelain

06 June 2013 6,492 0 View