I am looking for the method to determine bromelain enzyme concentration in unknown sample using HPLC C18 column. I would be very thankful and appreciative if anyone can help me with method and references.
Dear Mr. Adam, I am looking for a method for directly detecting the protein. Last time Type the type of column used was TSK Gel at UV detection at 280 nm for detection of bromelain enzyme, but now our column is broken and we only have C18 column in our lab thats why I am looking for the method for directly detecting the bromelain enzyme in my sample. Looking forward to hearing from you. Thanks a lot