For example,  MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide] can be used to stain fungi for the presence of cytoplasmic and mitochondrial dehydrogenases. If the fungal colony is viable, a purple precipitate (formazan) will be formed. If the colony is dead, it will not convert MTT to formazan.

Of course there are also many cell organelle-specific dyes that work well for many fungi and can be analyzed by fluorescence microscopy: DAPI, Hoechst (nuclear DNA), Mitotracker Red, Mitotracker Green FM, DASPMI (mitochondria), FM 4-64, MDY-64 (vacuole membranes), calcoflour (cell walls, septae), etc.

Best regards,

Christian

thank u sir

thank u sir

lactophenol cotton blue stain is good for identification mold

you can prepared according to Ellis (1994) from the following ingredients:-

Cotton Blue (Aniline Blue) 0.05 g

Phenol 2 g

Glycerol 40 ml

Lactic acid 20 ml

Distilled water 20 ml

Which were prepared by dissolving the cotton blue in the distilled water, then left to stand overnight to eliminate insoluble dye. The phenol crystals was added to the lactic acid in glass beaker, and with the aid of magnetic stirrer, the phenol was dissolved, then the glycerol was added, the cotton blue was filtered into the (phenol – glycerol – lactic acid) solution, after mixing, it was stored at room temperature to be used for staining and microscopic identification of fungi.

thank u

Lactophenol cotton blue blue is convenient for day to day use. It is known to be  most commonly used for microscopic study of fungi. For selective study you can make use of other strain as given by above workers as per your requirement.

Lactophenol cotton blue can be easily made in your lab or you can purchase ready made also like HighMedia (S016) and other suppliers.

yes we are already using

Lactophenl cotton blue is the best stain for felimintus fungi

PAS polysaccaride bright pink great screening stain

GMS silver technically problematic if too much reagent is used or faint if tissue substrate is large

Lactophenol cotton blue stain is used for identification mold

Can someone explain the pros and cons of Lactophenol cotton blue stain and PAS stain, please? Thanks in advance!

Lactophenolcotton Blue

Lactophenol cotton blue.

We have a lot of them., they are: 1) lactophenol cotton blue stain.

2) India ink stain/ nigrosin.

3) calcofluor white stain.

4) wright stain.

5) Hematoxylin stain.

6) Periodic acid Schiff (PAS) stain.