Dear,

GCMS, HPLC, NMR  are best and widely used methods for the characterization of metabolites. But if you want to know other methods, the answer is Preliminary methods for qualitative identification, IR, TLC, GLC. You can also read my papers.

Good Luck! 

I am also agree with Vinesh Kumar.

Depends on the type of secondary metabolite you are looking into - but I dont understand what you mean by -'good method' dont you think HPLC and GC coupled to a MS is good enough - and they are also relatively simple to use.  

yes i know both GCMS  and HPLC are widely used methods for identification compounds  still i want to try different methods .Thank u all for ur suggestions

Hi Bandarupalli Devadatha ,

Attached,

Article In-depth spectral characterization of antioxidative (1,3)-β-...

Article Efficient biomass-exopolysaccharide production from an ident...

Hope it helps

Best wishes

Wan