Bandarupalli Devadatha

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Questions related from Bandarupalli Devadatha

Which is the best method for extraction of DNA from Marine Fungi?

I had refered some literature about Fungal DNA extraction. I had seen that CTAB , Phenol chloroform , Kit methods are commonly used ones. So which will best one for extraction of DNA from Marine...

23 October 2015 5,324 2 View

During single spore isolation how many germinating spores are transfered from water agar into PDA?

While transfering germinating spores into pda instead of my desired fungi other fastidious fungi and bacteria is inhibiting the growth of desired fungi. How I can overcome this contamination. How...

19 June 2015 5,916 12 View

How do I overcome contamination during single spore isolation?

Currently I am working on single spore isolation method. some spores are germinating and some are non germinating.When i transfered the germinating spores into PDA they are contaminated with...

18 June 2015 9,188 13 View

Can anyone suggest the best method for isolation of marine fungi from twigs of mangrove plants?

some of the isolation methods like Single spore isolation , direct transfer, particle filtration can be followed .

18 June 2015 4,778 4 View

Can anyone suggest me a standard protocol for the estimation of a salinity of a sea water?

i have searched protocols for the estimation of a salinity of a sea water.but i need to know the widely used method please refer me good method

26 May 2015 6,808 5 View

Are there any methods to know antimicrobial activity of fungal cultures other than extraction and performing disc diffusion or well diffusion Assays?

It is very difficult to get Fungal Extract from many fungal cultures. I need to know whether any other methods to perform Antimicrobial microbial activity without extracting fungal extract. we can...

03 April 2015 7,577 13 View

Can anyone suggest a good article for Extraction identification and characterization of Secondary metabolites from Fungi?

I studied that ethyl acetate, hexane and methanol are best for extraction process. Are there any methods for identification of compounds apart from GCMS,HPLC,NMR? Can anyone suggest some good...

09 March 2015 5,681 5 View

What are the molecular identification methods used for fungi?

I had studied that internal transcribed spacer (ITS), Isolation of DNA and PCR amplification and DNA sequencing and BLAST and phylogenetic analysis are used. Can anyone suggest articles about...

04 March 2015 5,443 8 View

What are the cheapest and best methods for preservation of ascomycetes for a long time?

I had studied about freeze drying methods, mineral oil, distilled water, sterile soil, Silica gel. Among these methods which will be suitable method for preserving ascomycetes

04 March 2015 8,051 6 View

Which stains can be used for Staining Fungi?

Apart from Lactophenolcotton Blue, Haematoxylin, Melzers Reagent,Lactophenol

04 March 2015 3,170 14 View

Which solvents can be used for the extraction of hydrophobins and which concentration is require for the extraction?

The literature suggests that we have to add 2% SDS and triflouroacetic acid or formic acid can be used for extraction of hydrophobins. Is there any other solvent which can be used for this...

04 March 2015 8,051 2 View

What are the method used to induce sporulation of Ascomycetes On PDA?

I tried to induce sporulation by using sterile match sticks but thy are not sporulating Why they are Sporulating on Twigs as Host and why they are not Sporulating on PDA invitro

04 March 2015 1,475 5 View

Which are suitable methods to isolate fungi from decaying wood debris or twigs of mangrove forest?

I had worked following methods to isolate fungi Single spore isolation Direct isolation Serial dilution Is there any methods other than this? Please suggest a good articles regarding these methods.

04 March 2015 6,826 1 View

Can anyone suggest some keys for identification of marine and mangrove fungi in India?

Fungi colonizing on wood debris and twigs of Mangroove plants like Rhizopora sp Avicennia sp, sonnerita sp

04 March 2015 9,051 3 View