Which is the best method for extraction of DNA from Marine Fungi?

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During single spore isolation how many germinating spores are transfered from water agar into PDA?

While transfering germinating spores into pda instead of my desired fungi other fastidious fungi and bacteria is inhibiting the growth of desired fungi. How I can overcome this contamination. How...

18 June 2015 5,897 12 View

How do I overcome contamination during single spore isolation?

Currently I am working on single spore isolation method. some spores are germinating and some are non germinating.When i transfered the germinating spores into PDA they are contaminated with...

17 June 2015 9,170 13 View

Can anyone suggest me a standard protocol for the estimation of a salinity of a sea water?

i have searched protocols for the estimation of a salinity of a sea water.but i need to know the widely used method please refer me good method

25 May 2015 6,788 5 View

Are there any methods to know antimicrobial activity of fungal cultures other than extraction and performing disc diffusion or well diffusion Assays?

It is very difficult to get Fungal Extract from many fungal cultures. I need to know whether any other methods to perform Antimicrobial microbial activity without extracting fungal extract. we can...

02 April 2015 7,559 13 View

Can anyone suggest a good article for Extraction identification and characterization of Secondary metabolites from Fungi?

I studied that ethyl acetate, hexane and methanol are best for extraction process. Are there any methods for identification of compounds apart from GCMS,HPLC,NMR? Can anyone suggest some good...

08 March 2015 5,663 5 View

What are the method used to induce sporulation of Ascomycetes On PDA?

I tried to induce sporulation by using sterile match sticks but thy are not sporulating Why they are Sporulating on Twigs as Host and why they are not Sporulating on PDA invitro

03 March 2015 1,455 5 View

Which solvents can be used for the extraction of hydrophobins and which concentration is require for the extraction?

The literature suggests that we have to add 2% SDS and triflouroacetic acid or formic acid can be used for extraction of hydrophobins. Is there any other solvent which can be used for this...

03 March 2015 8,032 2 View

What are the molecular identification methods used for fungi?

I had studied that internal transcribed spacer (ITS), Isolation of DNA and PCR amplification and DNA sequencing and BLAST and phylogenetic analysis are used. Can anyone suggest articles about...

03 March 2015 5,416 8 View

What are the cheapest and best methods for preservation of ascomycetes for a long time?

I had studied about freeze drying methods, mineral oil, distilled water, sterile soil, Silica gel. Among these methods which will be suitable method for preserving ascomycetes

03 March 2015 8,031 6 View

Which stains can be used for Staining Fungi?

Apart from Lactophenolcotton Blue, Haematoxylin, Melzers Reagent,Lactophenol

03 March 2015 3,152 14 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Ashwini Kumar Dubey

http://download.springer.com/static/pdf/530/art%253A10.1385%252FMB%253A33%253A1%253A67.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1385%2FMB%3A33%3A1%3A67&token2=exp=1445618845~acl=%2Fstatic%2Fpdf%2F530%2Fart%25253A10.1385%25252FMB%25253A33%25253A1%25253A67.pdf%3ForiginUrl%3Dhttp%253A%252F%252Flink.springer.com%252Farticle%252F10.1385%252FMB%253A33%253A1%253A67*~hmac=cbdbf0d15cd0efa351006d086daed34cc168529c0d7ea3055dbe2a0d61387b20

Bandarupalli Devadatha

Thank u sir