I tried to induce sporulation by using sterile match sticks but thy are not sporulating
Why they are Sporulating on Twigs as Host and why they are not Sporulating on PDA invitro
Ascomycetes and Basidiomycetes produce characteristic spores after the
processes of plasmogamy, karyogamy, and meiosis. Heterothallism, which requires two
mating types, is more common than homothallism; therefore, zygospores, ascospores, and basidiospores are not commonly seen in the clinical laboratory because only one mating type is usually recovered.Ascomycetes are characterized by the development of sac-like cells called asci, which usually contain eight ascospores. Asci may form in a specialized fruiting body called an ascocarp. The development of the centrum is very important in distinguishing ascocarp types.Casein agar
casein, 10% acid hydrolyzed 25.0 ml
glucose 40.0 gm
Mg S04 0.1 gm
KH2
PO4 1.8 gm
agar 20.0 gm
distilled H O q.s. 1000 ml 2
adjust to pH 6.8
Ammonium nitrate agar can be used- Same as casein agar except substitute 1.5 gm NH N0 for 4 3 casein, 10% acid hydrolzyed
casien agar or Ammonium Nitrate agar can be used for sporulation in ascomycetes.Wether i can add oppsite strain so that sporulation may occur sir
Thank u sir
Leslie's suggestions are worth of consideration, also some ascomycetes prefer low PH environment.
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