Christian Q. Scheckhuber

9 Questions 445 Answers 0 Followers

Questions related from Christian Q. Scheckhuber

Some questions on steroid hormones. Can anyone help?

Steroid hormones are usually transported in the blood stream by carrier proteins. At their target cells they are released and can then either bind to surface receptors or diffuse through the...

22 March 2018 4,609 5 View

Can Amicon Ultra centrifugal filters be re-used?

I need to purify many samples using Amicon Ultra centrifugal filter devices (Millipore Merck). Does anybody know if it is possible to clean and re-use them? Thanks in advance!

29 September 2016 361 14 View

Is there any correlation between mitochondrial fission and fusion and mitochondrial DNA replication and transcription?

Mitochondria are dynamic organelles that continously fuse and divide. This behaviour is important for content mixing and mitochondrial quality control. I am interested whether mtDNA replication...

29 June 2016 8,908 4 View

Isn't it wrong to say that mitochondria generate or produce energy?

According to the principle of energy conservation it is not possible to create or destroy energy. Remarkably, PubMed database queries using the terms (mitochondria AND "energy generation") or...

20 June 2016 8,892 16 View

Is there a database or comprehensive list of glycated protein sequences?

I want to study which arginine or lysine residues in target proteins are preferentially modified by glycating agents like methylglyoxal. Is there a database or any other resource which lists the...

07 June 2016 3,550 3 View

Would monitoring pexophagy by ALP maturation assay work?

Transport of alkaline phosphatase (ALP) to the vacuole via autophagy and subsequent activation of its enzymatic activity by proteolysis has been used to measure rates of bulk autophagy (Noda et...

08 October 2015 7,687 1 View

Do you know a company that provides identification of protein modifications?

I have a known protein and subject it to glycation via methylglyoxal. After the reaction has been completed I would like to know whether Arg (Lys) residues have been modified by methlyglyoxal....

19 May 2015 5,923 4 View

Does anybody know how to measure TOR activity in filamentous fungi?

The TOR ('target of rapamycin') protein kinase is conserved from yeast to humans and controls cell growth in response to nutrient availability. I am planning to measure TOR activity in extracts...

14 January 2015 4,730 4 View

Is anyone familiar with the use of CMAC-based vacuole markers in filamentous fungi?

I recently tested the components of the "Yeast vacuole staining kit" from Life technologies for staining vacuoles in Penicillium chrysogenum. Carboxy-DCFDA and MDY-64 (both use the GFP...

26 November 2014 1,097 3 View