I had studied about freeze drying methods, mineral oil, distilled water, sterile soil, Silica gel. Among these methods which will be suitable method for preserving ascomycetes
The best storage method varies for different members of the Ascomycota. For a number of ascomycetes, particularly the faster growing ones, dried filter paper is a good and inexpensive method (see Peever and Milgroom. 1992. Phytopathology. 82:821-828.). Most fungi we have tried do well with the agar strip method. We have seen loss of virulence with plant pathogenic fungi stored in sterile soil and various morphological changes have been reported by others (e.g. http://128.104.77.228/documnts/pdf2004/fpl_2004_nakasone001.pdf).
Thank You for your suggestions i had gone through that article
dried filter paper or silicon gel would be ideal, especially for ascocarps that may sampled for DNAs. Glycerol would be good for cultures.
Store your cultures in sterile distilled water vials (2-3 ml). Try to transfer only mycelia and if available conidia. Do this in aseptic conditions, carefully. I observed that these tubes can be stored easily for two years in household refrigerator (4-10°C, Not freezer). This is a very cheap and efficient method.
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