Which solvents can be used for the extraction of hydrophobins and which concentration is require for the extraction?

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Which is the best method for extraction of DNA from Marine Fungi?

I had refered some literature about Fungal DNA extraction. I had seen that CTAB , Phenol chloroform , Kit methods are commonly used ones. So which will best one for extraction of DNA from Marine...

22 October 2015 5,310 2 View

During single spore isolation how many germinating spores are transfered from water agar into PDA?

While transfering germinating spores into pda instead of my desired fungi other fastidious fungi and bacteria is inhibiting the growth of desired fungi. How I can overcome this contamination. How...

18 June 2015 5,897 12 View

How do I overcome contamination during single spore isolation?

Currently I am working on single spore isolation method. some spores are germinating and some are non germinating.When i transfered the germinating spores into PDA they are contaminated with...

17 June 2015 9,170 13 View

Can anyone suggest me a standard protocol for the estimation of a salinity of a sea water?

i have searched protocols for the estimation of a salinity of a sea water.but i need to know the widely used method please refer me good method

25 May 2015 6,788 5 View

Are there any methods to know antimicrobial activity of fungal cultures other than extraction and performing disc diffusion or well diffusion Assays?

It is very difficult to get Fungal Extract from many fungal cultures. I need to know whether any other methods to perform Antimicrobial microbial activity without extracting fungal extract. we can...

02 April 2015 7,559 13 View

Can anyone suggest a good article for Extraction identification and characterization of Secondary metabolites from Fungi?

I studied that ethyl acetate, hexane and methanol are best for extraction process. Are there any methods for identification of compounds apart from GCMS,HPLC,NMR? Can anyone suggest some good...

08 March 2015 5,663 5 View

What are the method used to induce sporulation of Ascomycetes On PDA?

I tried to induce sporulation by using sterile match sticks but thy are not sporulating Why they are Sporulating on Twigs as Host and why they are not Sporulating on PDA invitro

03 March 2015 1,455 5 View

What are the molecular identification methods used for fungi?

I had studied that internal transcribed spacer (ITS), Isolation of DNA and PCR amplification and DNA sequencing and BLAST and phylogenetic analysis are used. Can anyone suggest articles about...

03 March 2015 5,416 8 View

What are the cheapest and best methods for preservation of ascomycetes for a long time?

I had studied about freeze drying methods, mineral oil, distilled water, sterile soil, Silica gel. Among these methods which will be suitable method for preserving ascomycetes

03 March 2015 8,031 6 View

Which stains can be used for Staining Fungi?

Apart from Lactophenolcotton Blue, Haematoxylin, Melzers Reagent,Lactophenol

03 March 2015 3,152 14 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View