Which is the most preferred bacterial species used in the biorefining of coal?

31 December 2013 1 2K Report

Biorefining of coal.

If you mean by "biorefining" the removal of sulfur/sulfide from coal ("biodesulfurication") then Acidithiobacillus (formerly Thiobacillus) species might be preferred oxidizing reduced sulfur compounds (e.g., pyrite) to sulfate.

Badges
Science method

More Srujana Kathi's questions See All

Is polybrene for lentiviral transduction toxic to human fibroblasts? If you use it, what working concentration do you suggest (5-8 ug/mL)?

I would like to enhance transduction efficiency in a human fibroblast line. Do you have experience with using polybrene or would you suggest protamine sulphate? What is the usual working...

30 May 2023 5,396 5 View

How much can we reduce the off-target effects of genome editing?

the frequency of off-target effect varies among the genome-editing technologies. TALENs produce the fewest off-target edits because they use a longer stretch of target DNA than ZFNs or CRISPR-Cas9

06 August 2021 5,041 2 View

What would happen if recombinant microbes could transfer genetically altered genes into other microorganisms which they were not originally intended?

What would happen if recombinant microbes could transfer genetically altered genes into other microorganisms for which they were not originally intended? should we be concerned?

30 July 2021 7,805 3 View

Have you ever experienced problems in dissolving resorufin beta-glucopyranoside?

We want to set up an activity assay for beta-glucocerebrosidase. We are experiencing difficulties in fully dissolving resorufin beta-glucopyranoside. We tried it in acetate buffer, methanol and...

29 May 2020 8,492 0 View

What is the HEPES buffer concentration to be used for MCF 10A cell culture without 5% CO2 supply for 6-8 hours?

Hi, I am doing experiments with MCF 10A cells, which were cultured in DMEM/F12 supplemented with 5% horse serum, 20ng/ml of EGF, 0.5µg/ml of hydrocortisone, 100ng/ml of cholera toxin, 10µg/ml of...

26 February 2020 4,025 0 View

What are the advantages and disadvantages of RealTime-Glo vs CellTiter-Glo Cell Viability Assay? Which reagent is more suitable for which application?

We would like to assess cell viability and denisity in a 384 well-plate format.

26 January 2020 4,100 2 View

Conversion of acceleration time history to displacement time history ?

I have the time history acceleration data with me. I got the velocity data from the traditional integration method - Trapezoidal. Again I integrated the velocity signal to get displacement. I got...

21 January 2020 6,189 1 View

What is the optimal concentration and incubation of MG132 in HEK293 cells?

I would like to test whether the stability of my protein of interest is increased upon inhibition of proteasomal degradation. I transiently transfect HEK293 cells with my protein of interest (a...

10 April 2019 3,831 5 View

Is there a way to measure the exact amount of heat trapped by green house gases?

in case of green house gases like carbon di oxide is it possible to measure the exact amount of heat trapped by a certain amount of CO2 in a confined space and be able to tell the temperature rise?

27 November 2018 1,576 1 View

Have you found that there are people who suffer from chronic pain but do not wish to take narcotics?

Hello, I am a 57 year old female who is morbidly obese and have suffered three strokes on the right side. I suffer from chronic pain and numbness. Not only on my right side but my lower back as...

13 June 2017 1,033 6 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

How can I find access to heavy metal reference doses from the EPA and WHO websites?

Reference dose and Maximum acceptable concentrations HMs

03 August 2024 8,230 4 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

Posthoc test lettering in JAMOVI?

Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...

31 July 2024 3,333 4 View

Given the current level of natural phenomena cause by the climate change and environmental pollution, will the AI find the technological solutions?

AI-driven technologies offer previously unheard-of capabilities to process enormous volumes of data, extract insightful knowledge and improve predictive models, according to the UN’s World...

31 July 2024 9,733 3 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View