We want to set up an activity assay for beta-glucocerebrosidase. We are experiencing difficulties in fully dissolving resorufin beta-glucopyranoside. We tried it in acetate buffer, methanol and DMSO, but nothing worked. Thank you!
I would like to enhance transduction efficiency in a human fibroblast line. Do you have experience with using polybrene or would you suggest protamine sulphate? What is the usual working...
30 May 2023 5,396 5 View
the frequency of off-target effect varies among the genome-editing technologies. TALENs produce the fewest off-target edits because they use a longer stretch of target DNA than ZFNs or CRISPR-Cas9
06 August 2021 5,041 2 View
What would happen if recombinant microbes could transfer genetically altered genes into other microorganisms for which they were not originally intended? should we be concerned?
30 July 2021 7,805 3 View
We would like to assess cell viability and denisity in a 384 well-plate format.
26 January 2020 4,100 2 View
I would like to test whether the stability of my protein of interest is increased upon inhibition of proteasomal degradation. I transiently transfect HEK293 cells with my protein of interest (a...
10 April 2019 3,831 5 View
Hello, I am a 57 year old female who is morbidly obese and have suffered three strokes on the right side. I suffer from chronic pain and numbness. Not only on my right side but my lower back as...
13 June 2017 1,033 6 View
When HCL was added into the yeast tank, foaming starts to form aggressively and gets thicken when pH drops to around 5.4-5.6. The appearance of bubbles is different to that before HCL was added....
12 January 2017 8,470 6 View
Looking for published research on the motivation behind and the definition of the stages a patient goes through, moving from patient to patient advocate to expert patient advocate.
26 June 2015 916 7 View
eigenvalues and eigenvectors
27 April 2015 9,378 8 View
Working on project proposal for mentoring program for African American women in leadership both in church and community. Searching for information on challenges faced
20 January 2015 1,398 5 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View