How much can we reduce the off-target effects of genome editing?

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How do I calculate potency of a working standard for assay evaluation of a Tablet formulation by RP-HPLC?

I have come across a formula to calculate the amount of drug present in a tablet formulation that mentions as such Amount of drug = (Peak area of sample/Peak area of standard) * (Dilution factor...

13 December 2020 4,224 3 View

How to represent Input data as multinomial distribution of feature vectors?

Hello everyone, Is there any approach to generate a fixed no. of feature vectors from the sequential data(similar to time series data...) and also express the same sequential data in terms of the...

29 November 2020 5,508 3 View

How to solve the HAZE problem in Low emissivity coatings ?

Dear friends, I am facing a problem with an issue of milky Haze in the coatings developed for aesthetic applications. All the layers were deposited by magnetron sputtering having thickness in...

08 November 2020 2,853 3 View

How to design double layer silver low emissive coating?

Dear friends of the research community, I am having an issue with double-layer low emissive coating fabrication. the coating of silver layer is not been adhere to the glass surface in this...

04 August 2020 7,730 3 View

How and what kind of coating designs are used in architectural glass?

Hi friends, research scholar, professors, Can anyone help in the design of coatings for architectural glass which is used for buildings?

22 July 2020 1,063 3 View

Is the antenna designed for Ku band operating at 14.25GHz suitable for terrestrial communication? or suitable for RADAR communication?

I have designed an antenna to operate at 14.25 GHz( 14 to 14.5 GHz)uplink frequency of Ku band with linear polarization. what kind of application is suited for my designed antenna? and reference...

11 July 2020 699 2 View

Anyone Scientist recorded the recovery of marine environment after ths pandamic lockdown?

Marine environemnt faced lot of issues like pollution and biodiversity loss due to industrialization and other ocean related activities. We should think seriously about the marine conservation for...

29 May 2020 5,270 1 View

Can we find the force of the collision by analyzing the sound of the collision ?

I'm curious to know if one can find the force of the collision by analyzing the audio or extracting features from the audio signal that estimates the force.

14 May 2020 3,981 3 View

How to define hyper elastic UMAT in a UEL?

Hi all, I am working on Phase-Field UEL for hyperelastic materials. I am using codes of Dr Molnar ( http://www.molnar-research.com/tutorials_videos_3.html) to develop hyperelastic material. I...

07 April 2020 4,243 1 View

What is the major difference between analyzing the elemental composition of fish otolith using EDS and ICPMS or ICPOES?

Here, I mean the accuracy and the major differences.

23 February 2020 5,798 8 View

In what ways do fellow reaerchers think that mRNA vaccination may be under CRISPR systems technology application ?

There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...

18 February 2021 9,109 5 View

How can I completely delete a protein on the genomic level using Crispr?

I want to completely delete or largely truncate a gene and not only achieve a functional knockout in a cell line. I can only transfect my cells with the Crispr/Cas9 sgRNA RNP and not introduce a...

08 February 2021 2,032 2 View

Choise of cell line for neurobiology?

I am working with myelin-related proteins and interested to do cell experiments with my target protein in cells. I have created lentiviruses for my proteins, and mutants and also CRISPR set-up for...

02 February 2021 8,083 1 View

Does gene insertion via CRISPR/Cas9 result in chromosomal abnormalities?

Hello, I hope to insert a gene into mammalian cells in culture using CRISPR/Cas9 and an HDR template. Will insertion of a 1 kilobase gene cause chromosomal abnormalities and effect cell...

31 January 2021 2,516 1 View

Does anyone has a protocol for transfecting AML12 cells?

I am optimizing a CRISPR/CAS9 system in vitro before using it in vivo. I would like to transfect AML12 cells with my pAAV-sgRNA-vector to validate editing efficiency before generating virus.

26 January 2021 8,733 2 View

Should I add a promoter upstream of my CRISPR-inserted gene?

Hello, I want to insert a gene into my cells using sgRNA, Cas9, and an HDR template. The gene that I will insert into has its own promoter. Because I will cut the gene downstream of its start...

14 January 2021 1,170 2 View

Does anyone have problems performing CRISPR KO in human primary T cells?

For the past few months, I have been troubleshooting a CRISPR KO of human CD3e in human primary T cells. I got the KO to work one time using 1250 ng TrueCut Cas9 (Thermo) + 250 ng (or 7.5 pmol)...

11 January 2021 6,016 3 View

After CRISPR and single-colony picking, cells reverted to WT?

Hi guys: I have been doing CRISPR knock-in and knock-out and one seemingly impossible scenario keeps happening. In the CRISPR knock-in attempt I tried to add a tag on a gene of interest, so I...

07 January 2021 190 3 View

How to choose mouse cell line to work on?

Hey, I am looking to start working on mouse cell line for some genomics experiments. I have the following requirements: 1) Normal chromosomes composition. 2) Possible to transfect. 3) Possible to...

03 January 2021 4,387 2 View

Which is the best gene editing tool among TALENs, ZFNs, CRISPR and HENs?

Since two decades we came across many tools to edit the desired gene and genome viz., clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9), (2)...

27 December 2020 7,667 10 View