How to do co culture of human fibroblast and breast cancer cells ?

Hi all, I want to perform the co culture of human dermal fibroblast and breast cancer cells. I have collected conditioned media from cancer cells and treated on fibroblasts and observed the...

10 November 2017 3,628 1 View

Why fibroblasts Change morphology after one/two passages ?

Hello, I am working on human dermal fibroblasts. After thawing from liquid nitrogen, there is clear morphology till one/two passages. But after that we observe a change in morphology, like the...

02 March 2017 1,131 6 View

What causes dark zone in agarose gel ?

I ran a 1 % agarose gel with Ethidium Bromide to detect DNA. When analysing under UV I see bands but I also have a very dark zone impeding visualization of the migration What causes dark zone in...

02 March 2017 9,465 1 View

Can anyone please suggest a normal breast-associated fibroblasts (NAF) and Breast carcinoma associated fibroblast(CAF) cell line..??

Hello friends, Can anyone please suggest a normal breast-associated fibroblasts (NAF) and Breast carcinoma associated fibroblast(CAF) cell line..?? One more query, I have HaCat cell line, can it...

07 August 2016 6,985 1 View

How much PBS should I dilute for preserving exosomes. ?

07 August 2016 8,322 5 View

How to quantify Exosomes ?

I have isolated exosomes from conditioned cell culture media . I have to go for its downstream applications and need to do the MTT assay. For that how to quantify the exosomes. Can we go with...

02 March 2016 4,466 12 View

Can we use incomplete media for exosome isolation ??

Instead of using exosome free FBS in complete media, if we go for incomplete media (after culturing cells in complete media and then washing with incomplete media and flooding the culture vessel...

02 March 2016 936 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View