Hello friends, Can anyone please suggest a normal breast-associated fibroblasts (NAF) and Breast carcinoma associated fibroblast(CAF) cell line..??
One more query, I have HaCat cell line, can it be of any use in relation to above cell lines...??
Primary culture of breast cancer-associated fibroblasts are commercially available. However, they are not established cell lines.
Hi all, I want to perform the co culture of human dermal fibroblast and breast cancer cells. I have collected conditioned media from cancer cells and treated on fibroblasts and observed the...
10 November 2017 3,717 1 View
Hello, I am working on human dermal fibroblasts. After thawing from liquid nitrogen, there is clear morphology till one/two passages. But after that we observe a change in morphology, like the...
02 March 2017 1,196 6 View
I ran a 1 % agarose gel with Ethidium Bromide to detect DNA. When analysing under UV I see bands but I also have a very dark zone impeding visualization of the migration What causes dark zone in...
02 March 2017 9,561 1 View
Hello Everyone, I'm going to isolate exosomes using ExoQuick from 10 ml of culture media, by using 1/5 amount of the kit. After isolation how much PBS should I dilute to preserve the exosomes.
07 August 2016 8,388 5 View
I have isolated exosomes from conditioned cell culture media . I have to go for its downstream applications and need to do the MTT assay. For that how to quantify the exosomes. Can we go with...
02 March 2016 4,541 12 View
Instead of using exosome free FBS in complete media, if we go for incomplete media (after culturing cells in complete media and then washing with incomplete media and flooding the culture vessel...
02 March 2016 1,006 2 View
Which one is better to go for Exosome isolation from cell culture media in terms of it's yield ? Exoquick from system biosciences or Total exosome isolation reagent from ThermoFisher sc....?? Yes,...
01 February 2016 6,821 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View