Why fibroblasts Change morphology after one/two passages ?

Hello, I am working on human dermal fibroblasts. After thawing from liquid nitrogen, there is clear morphology till one/two passages. But after that we observe a change in morphology, like the...

02 March 2017 1,131 6 View

What causes dark zone in agarose gel ?

I ran a 1 % agarose gel with Ethidium Bromide to detect DNA. When analysing under UV I see bands but I also have a very dark zone impeding visualization of the migration What causes dark zone in...

02 March 2017 9,465 1 View

Can anyone please suggest a normal breast-associated fibroblasts (NAF) and Breast carcinoma associated fibroblast(CAF) cell line..??

Hello friends, Can anyone please suggest a normal breast-associated fibroblasts (NAF) and Breast carcinoma associated fibroblast(CAF) cell line..?? One more query, I have HaCat cell line, can it...

07 August 2016 6,985 1 View

How much PBS should I dilute for preserving exosomes. ?

07 August 2016 8,322 5 View

How to quantify Exosomes ?

I have isolated exosomes from conditioned cell culture media . I have to go for its downstream applications and need to do the MTT assay. For that how to quantify the exosomes. Can we go with...

02 March 2016 4,466 12 View

Can we use incomplete media for exosome isolation ??

Instead of using exosome free FBS in complete media, if we go for incomplete media (after culturing cells in complete media and then washing with incomplete media and flooding the culture vessel...

02 March 2016 936 2 View

Which is the better option for Exosome Isolation ..??

Which one is better to go for Exosome isolation from cell culture media in terms of it's yield ? Exoquick from system biosciences or Total exosome isolation reagent from ThermoFisher sc....?? Yes,...

01 February 2016 6,752 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View