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Questions related from Bikash Chandra Jena
Hi all, I want to perform the co culture of human dermal fibroblast and breast cancer cells. I have collected conditioned media from cancer cells and treated on fibroblasts and observed the...
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Hello, I am working on human dermal fibroblasts. After thawing from liquid nitrogen, there is clear morphology till one/two passages. But after that we observe a change in morphology, like the...
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I ran a 1 % agarose gel with Ethidium Bromide to detect DNA. When analysing under UV I see bands but I also have a very dark zone impeding visualization of the migration What causes dark zone in...
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Hello friends, Can anyone please suggest a normal breast-associated fibroblasts (NAF) and Breast carcinoma associated fibroblast(CAF) cell line..?? One more query, I have HaCat cell line, can it...
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Hello Everyone, I'm going to isolate exosomes using ExoQuick from 10 ml of culture media, by using 1/5 amount of the kit. After isolation how much PBS should I dilute to preserve the exosomes.
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Instead of using exosome free FBS in complete media, if we go for incomplete media (after culturing cells in complete media and then washing with incomplete media and flooding the culture vessel...
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I have isolated exosomes from conditioned cell culture media . I have to go for its downstream applications and need to do the MTT assay. For that how to quantify the exosomes. Can we go with...
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Which one is better to go for Exosome isolation from cell culture media in terms of it's yield ? Exoquick from system biosciences or Total exosome isolation reagent from ThermoFisher sc....?? Yes,...
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