Which is a better choice for precipitation of plant genomic DNA: chilled ethanol or isopropanol and why?
Chilled Ethanol (95%) is always better to precipitate Genomic DNA.
Ethanol is more strong precipitator than isopropanol, but if you use for exaple Doyle&Doyle protocol for DNA isolation, you use both on different stages of this process. As final stage of DNA precipitation ethamol traditionally used.
Based on my experience, I prefer ethanol. The advantage of isopropanol is that you need a smaller volume, in comparison with ethanol, to precipitate nucleic acids. Also, be aware that using it cold (chilled) could favor the co-precipitation of carbohydrates.
I feel isopropanol is better than ethanol for precipitation. we get better quality of DNA
Agree with Leandro. Personally, I do not like this isopropanol crap, and it is only a matter of volume.
Furthermore, if I remember correctly, the "cold" effect has been extensively reviewed...
If you use pre-chiled Ethanol 70% is as effective as isopropanol. But remember you need the use cold ethanol.
The other things is the volum in which you have the DNA that you want to precipitate. As it has been said before, isopropanol advantatge is the reduced volum you need.
isopropanol if precipitation of nucleic acids at room temperature
I use Isopropanol and it works great! 30min at-20C, or 5 min at-80C gives good precipitation.
I use isopropanol and it gives good precipitation of gDNA at -20C for 1-2hr.
Isopropanol is excellent, and has the benefit that you can use smaller amounts than ethanol if volume is an issue
Hi there
I guess you should use isopropanol for the initial precipitation as it is a very potent alcohol for such proceedures. You should though afterwards wash with 70% ethanol for the reasons of extra desalting an also because isopropanol may provide you with a bit more volatile pellet, so a wash with ethanol would "stabiiise" your pellet at the ottom of the tube.
Nikos
We use Edward's protocol to isolate genome DNA and the precipitation is done with isopropanol just for 2 minutes at room temperature.
2 volume ethanol (room temperature) would be the best rather than chilled and isopropanol..One disadvantage of isopropanol is that it co-precipitates carbohydrates during storage at low temperature..!!
Isopropanol will help a lot During isolation of DNA , It will help in precipitation and it was a good precipitating agent too compare to ethanol
While Ethanol we can use in Sugars Extraction.
Thanks
Choice of ethanol or isopropanol is often a question of volume added. For ethanol you must add aroud 2V of alcool, for isopropanol, just 1V. When there's no place enough in your microtube for ethanol, you can choose isopropanol. Both work well, but precipitation by isopropanol need a addiditive washing with ethanol, because of trace of isopropanol that could remain in your DNA.
Sincerely
You can either use isopropanol or absolutel ethanol for precipitation & beth work fine. For washing the pellet and removing salts use 70% ethanol.
Dear Dr. Arvind Kumar Goyal
Personally, I prefer isopropanol. Generally I use it at room temperature after wash with EtOH 70% and then, I incubate it for a few hours at -80 °C before precipitating.
Here I attached you a useful guide for the selection of alcohols and salts for nuclei acid precipitation.
Good luck, and best regards from Argentina!!
Rosalia
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