Maulik Rachh

17 Questions 44 Answers 0 Followers

Questions related from Maulik Rachh

How to convert HIV Copies/mL to IU/mL ?

I am developing HIV Viral Load Detection Assay, in that my Quantitative Standards are in Copies/mL, but I want to convert it to International Unit (IU)/mL as WHO recommendation to give results in...

28 September 2019 6,277 7 View

Problem in HIV RT PCR, every samples and water also coming positive at same time. Can anyone suggest what is the problem.?

I am using primer & Probe concentration 10 uM and using 0.5 uL in 25 uL reaction PCR setup. I have used so many different water but each water coming positive. Its might be primer dimer but...

06 February 2019 298 10 View

Which stabilizer we can used to stabilized RT PCR Master Mix?

I have developed RT PCR mastermix , now its required to add stabilizer in it? if yes which stabilizer we can use at which concentration.

03 December 2018 1,733 9 View

Which Gene Internal Control Best for DNA Real Time PCR?

I want to amplify DNA with Internal Control

22 August 2018 492 3 View

What is the best way to design DNA/RNA Extraction control for RT PCR testing?

I want to know the artificial gene or strain which can be use as a DNA/RNA extraction control, and will lead you to RT PCR testing for any viral DNA or RNA.

11 April 2018 8,477 4 View

Can any one know stabilizer for PCR Master Mix?

I want to stabilize the RT PCR Master Mix at Room Temperature . Please suggest if any one having same experience or some stabilizer i can try.

07 October 2017 9,433 3 View

What is the importance of GGGGS linker for Fusion protien?

Whay Gly-Gly-Gly-Gly-Ser linker mostly used as a linker for protien? Does its not interfere protein structure?

31 January 2015 9,735 6 View

Can anyone Provide me the protocol for Real Time PCR for MicroRNA Amplification and detection?

I want to perform RT PCR for MicroRNA Identification. Please provide me the protocol for the same.

08 December 2014 6,427 1 View

How much primer and probe concentration are required for optimization of RT PCR for tuberculosis?

I am working on diagnosis of tuberculosis with Taqman Probe in RT PCR. But sometimes negative also comes positive & sometimes positive also comes negative. I am using Primer & Probe...

11 October 2014 5,121 2 View

How can I optimize real time PCR?

Can any one help me to describe step by step process?

13 August 2014 1,600 2 View

Does anyone have experience with DNA Isolation from sputum samples?

Ratio of 260/280 ~ 1.80, is really much matter for PCR or RT PCR ? Or ~2.00 will also works for the same.

04 August 2014 2,177 3 View

Are intronic microRNAs a reaction mechanism or a defence mechanism of the body?

If microRNA comes from intervening sequences, its reaction of any mechanism or its itself generated body defence mechanism? Means its reaction of body process in disease creation stage or cellular...

15 July 2014 5,921 4 View

Why is there no amplification in Real Time PCR?

I am performing RT PCR for detection of tuberculosis. I am using Taqman Probe. I am not getting amplification. There is no curve line which cross the threshold line even in a positive sample.

17 April 2014 1,817 17 View

Why am I getting false positive result in -ve when working with LAMP?

I am working with LAMP (Loop-Mediated Isothermal Amplification) technology for the diagnosis of Tuberculosis. Why am I getting false positive result in -ve? We tried all new buffers and primers...

19 March 2014 2,354 19 View

Is isolation of DNA from a sputum sample without centrifugation possible? How?

I want to obviate the centrifuge steps for DNA Isolation.

03 December 2013 9,903 1 View

If we found same MicroRNA Expressing for Two Different Cancers, Can we use the same MicroRNA for Diagnosis of Both the Cancer?

16 April 2013 7,796 6 View

Which is the best protocol for Antibody Gold Conjugation?

Gold Conjugation Preparation Protocol

01 January 1970 8,979 5 View