19 Questions 46 Answers 0 Followers
Questions related from Maulik Rachh
I want to synthesized one Probe from one publish article, but its showing like "T" in between the sequence? Can anyone knows what is the meaning for the same? [6FAM]...
10 July 2020 9,236 0 View
I am developing HIV Viral Load Detection Assay, in that my Quantitative Standards are in Copies/mL, but I want to convert it to International Unit (IU)/mL as WHO recommendation to give results in...
28 September 2019 6,327 7 View
I am using primer & Probe concentration 10 uM and using 0.5 uL in 25 uL reaction PCR setup. I have used so many different water but each water coming positive. Its might be primer dimer but...
06 February 2019 374 10 View
I have developed RT PCR mastermix , now its required to add stabilizer in it? if yes which stabilizer we can use at which concentration.
03 December 2018 1,811 9 View
I want to amplify DNA with Internal Control
22 August 2018 577 3 View
I want to know the artificial gene or strain which can be use as a DNA/RNA extraction control, and will lead you to RT PCR testing for any viral DNA or RNA.
11 April 2018 8,561 4 View
I want to stabilize the RT PCR Master Mix at Room Temperature . Please suggest if any one having same experience or some stabilizer i can try.
07 October 2017 9,500 3 View
What blocking buffers can I use for a Lateral Flow Immunoassay? (NCM Blocking, after spraying)
29 July 2015 3,559 2 View
Whay Gly-Gly-Gly-Gly-Ser linker mostly used as a linker for protien? Does its not interfere protein structure?
31 January 2015 9,791 6 View
I want to perform RT PCR for MicroRNA Identification. Please provide me the protocol for the same.
08 December 2014 6,489 1 View
I am working on diagnosis of tuberculosis with Taqman Probe in RT PCR. But sometimes negative also comes positive & sometimes positive also comes negative. I am using Primer & Probe...
11 October 2014 5,169 2 View
Can any one help me to describe step by step process?
13 August 2014 1,651 2 View
Ratio of 260/280 ~ 1.80, is really much matter for PCR or RT PCR ? Or ~2.00 will also works for the same.
04 August 2014 2,220 3 View
If microRNA comes from intervening sequences, its reaction of any mechanism or its itself generated body defence mechanism? Means its reaction of body process in disease creation stage or cellular...
15 July 2014 5,973 4 View
I am performing RT PCR for detection of tuberculosis. I am using Taqman Probe. I am not getting amplification. There is no curve line which cross the threshold line even in a positive sample.
17 April 2014 1,873 17 View
I am working with LAMP (Loop-Mediated Isothermal Amplification) technology for the diagnosis of Tuberculosis. Why am I getting false positive result in -ve? We tried all new buffers and primers...
19 March 2014 2,410 19 View
I want to obviate the centrifuge steps for DNA Isolation.
03 December 2013 9,948 1 View
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16 April 2013 7,839 6 View
Gold Conjugation Preparation Protocol
01 January 1970 9,028 5 View
