4 Questions 11 Answers 0 Followers
Questions related from Muhammad Ansar Farooq
Hello, To check specificity of your primers by ncbi blast, we find identity percentage, query cover and E-value as the parameter to be taken care off. Which parameter is the most important and...
04 August 2014 6,898 4 View
Hi, I am facing problem of variable results in pcr. Despite the fact that I am adding each reaction component very carefully, I do face the problem again and again. I checked properties of my...
04 August 2014 7,465 8 View
Hi, I put my PCR tubes in and forgot to press enter in order to start it. After 1 hour when I was expecting that it would be finished I went and checked that it was never started. Has anyone made...
24 July 2014 9,793 41 View
Hi! I am using plant samples taken at different time points after stress to check the response of stress responsive genes but i am shocked that the band intensity of those targets is always same...
16 July 2014 4,671 2 View