Muhammad Ansar Farooq

4 Questions 11 Answers 0 Followers

Questions related from Muhammad Ansar Farooq

Primer designing: What is the importance of identity, query cover, and E-value?

Hello, To check specificity of your primers by ncbi blast, we find identity percentage, query cover and E-value as the parameter to be taken care off. Which parameter is the most important and...

04 August 2014 6,896 4 View

Does primer self annealing property cause variable PCR results?

Hi, I am facing problem of variable results in pcr. Despite the fact that I am adding each reaction component very carefully, I do face the problem again and again. I checked properties of my...

04 August 2014 7,463 8 View

What would happen if one forgot to start PCR after putting samples in it?

Hi, I put my PCR tubes in and forgot to press enter in order to start it. After 1 hour when I was expecting that it would be finished I went and checked that it was never started. Has anyone made...

24 July 2014 9,792 41 View

Why are the band intensities of markers the same under control and stress taken at different time points?

Hi! I am using plant samples taken at different time points after stress to check the response of stress responsive genes but i am shocked that the band intensity of those targets is always same...

16 July 2014 4,670 2 View