NILESH KUMAR SHARMA

6 Questions 24 Answers 0 Followers

Questions related from NILESH KUMAR SHARMA

Is anyone familiar with enzymatic saccharification of biomass?

Dear All, I am performing saccharification of biomass with the help of commercial cellulase enzyme. But when I am treating biomass with enzyme and taking reading at 0 hr it shows a pick of sugar...

03 January 2015 3,194 17 View

Can anyone tell me how to perform protoplast fusion of yeast cells?

Dear all, I want to transform yeast cell by protoplast fusion technique. So please provide me a protocol and suggest me any kit, if available. This technique is completely new for me, so your...

03 January 2015 4,723 2 View

What concentration of NAD and Tris Cl should I use for ADH enzyme assay?

I am performing enzyme assay for ADH. I am confused with what concentration of NAD and Tris Cl can be used. If any one has a protocol, please provide it for me.

10 July 2014 8,255 4 View

Is ethanol a growth associated product or non growth associated product?

Ethanol production starts at log phase then it is primary a product but it also increases at the stationary level. So which type of product is it?

15 May 2014 5,203 4 View

How can I identify hexose and pentose transporters in yeast?

Is there any alternative method for identification of pathway other than gene knockout?

04 December 2013 5,096 5 View

Protocol for D. hansenii transformation

Hi Folks, I am looking for the efficient protocol to transform D. hansenii either chemical transformation or electroporation. I have already tried a protocol described by Minhas et al. 2009...

01 January 1970 7,441 3 View