Some manufacturers supply variety of Fetal bovine sera (FBS) with same composition but they are sterilized by different methods such as Heat inactivated and Gamma irradiated ?
I would like to know which one is effective and does not effect the growth of cells especially human cancer cell lines when cultured in DMEM medium.
waiting for your valuable suggestions. Thanks in advance
In our labs we use heat inactivated. We are culturing colorectal carcinoma cell lines and it's doing well
We too heat inactivate our FBS with all of our cell lines, cancer, mouse and human.
We maintain the FBS in our complete medium at 10% v/v
My Heat Inactivation Protocol:
1. Thawl from frozen in 37C
2. Warm to 56-60C for 30 minutes
3. Cool in ice bath for 10 minutes
4. Aliquot into 50mL tubes sterial in hood
5. Store at -20C, Labled FBS H/I (Heat Inactiveated)
In our labs we use heat inactivated FBS
It's better to culture human cancer cells in DMEM + 10% heat inactivated FBS + 0.1%Penstrap.
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