Why do we need to give low pressure, high temperature and inert gas environment during synthesis of polymer?

I am working in synthesis of material.

14 December 2020 8,092 5 View

RS dataset of ice cover of Arctic Antarctic?

I am looking for datasets of ice cover of Arctic Antarctic. I want only India related dataset. If you have any source of dataset please share with me. It will be very helpful for my...

13 October 2020 7,137 1 View

How can I false color of SEM images (tiff format) ?

I have SEM image of cross section of multilayer thin film sample and need to represent the different color for different layer without losing its morphological information on the image. Is there...

08 October 2020 9,764 6 View

How to model deformable metal powder?? How to define gap between the particles?? Which modeling software can be used??

We need to model deformable metal powder. It will be further used in simulation process.

30 September 2020 9,329 7 View

Can we use homoeopathic high potency medicine for plant growth and development for their nutritional purpose?

The homoeopathic medicine consist minerals in it and the same nutrients are essential for the growth and development of the plant. So I asked myself whether the homoeopathic medicine useful for...

19 September 2020 8,339 10 View

When the Renewable Energy Sources integrated to the main grid, the with and without RE sources, how the system characteristics performance?

While incorporating the DER to the conventional grid, then what will be the effect of system stability and frequency

07 September 2020 2,386 7 View

Can anybody suggest how to prepare monosilicic acid from any source of silicon?

Monosilicic acid is the available form in which plant can take silicon and this is not a stable form. That's why I want to know how to prepare that and how to estimate that. Kindly give your inputs.

01 July 2020 6,226 3 View

Should we adopt the latest tools of Precision agriculture immediately? If not now, then when?

Precision agriculture (PA), in India, is synonymous to Remote sensing, GIS, GPS, VRA and VRT. These are old technologies and during last 3-4 decades enough research has been carried out. But the...

11 March 2020 8,581 15 View

Can we differentiate aerobic microbes from anaerobic microbes while isolation?

Just wants to know whether we can differentiate aerobic and anaerobic bacteria from same isolation method or we have to follow another method of estimation for anaerobic bacteria.

07 February 2020 7,351 5 View

How long the traditional nutrient management trials in crops will be continued with graded levels of fertilizer?

During last 5-6 decades we have generated enough data from traditional experiments on nutrient management with graded levels of fertilizers in almost all systems and situations. Research on...

04 February 2020 7,451 3 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Defining effective diffusivity in porous zone and non-porous zone?

Hi all, I am working on methanol steam reforming using packed bed for hydrogen generation. I am using FLUENT software for CFD simulation. In reference paper, authors mentioned that effective mass...

10 February 2021 6,104 1 View

Online Shopping Security Survey?

I would appreciate if you answer my DBA research questionnaire. Thank you all in...

10 February 2021 1,990 3 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

Can any one provide information about medicine, epidemic and critical issues or it is mandatory to be an expert ?

Due to the pandemic of coronavirus, the most source that the people depend on was social media. There is no guaranteed solution that is biased from political opinion. An example of that the...

01 February 2021 7,567 3 View

Can I use 500ng of DNA for restriction digestion?

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...

31 January 2021 729 6 View

Can't run my IPMSM drive system with DC-supply higher than 125 V. Have anyone had similar problem?

Hey, I try to run my 4 pole IPMSM with PWSVM and use a typical controller with PI-controller with a current loop and speed loop. When I run my system with DC-supply 100 V with a steady-state speed...

28 January 2021 7,354 8 View

How can I solve this problem with agarose gel DNA purification of bigger (1-4kb) fragments ?

Hi everyone, I perform a PCR + Restriction Digestion (single cut, same at both sides) over a metagenomic library. After that, I am trying to purifiy DNA fragments between 1-4kbp long from the...

24 January 2021 2,190 5 View