Hello, RG Members, I have designed the COBRA primers using the UCSC genome browser for the promoter regions of genes of my intreset. are these primers reliable? I have tried the other softwares like MethPrimer. MethPrimer 2.0. resluting in bad connection ( not able to access). MSP-HTPrimer also resulting failure. just wanted to know if the designed primers from Primer3 are reliable enough to before oredering them.

for COBRA can i use single restriction enzyme for all the genes of my intreset. How can I go for the mutiple genes DNA Methylation stduy by COBRA? what are the possible and easiest ways to carry out this technique? Will be waiting for the replies of this community.

Thanks in advance

Sagar N

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