Emile Alghoul

4 Questions 8 Answers 0 Followers

Questions related from Emile Alghoul

Is it necessary for a Halo-Tag to be free or can it be internal?

I am adding a Halo-Tag to a protein that is already tagged at its N-terminal. I prefer my tags to be just at one side to leave the C-ter free. I plan to add the Halo between the two. Will this...

22 March 2021 337 0 View

Does tissue lysis by sonication affect the RNA integrity?

I am extracting RNA from FFPE tissue (Phenol Chloroform method). I am not being able to achieve complete homogenization with the tissue homogenizer. I was thinking about using sonication. Do you...

18 December 2016 297 1 View

Does Qiazol enables the extraction of proteins and DNA from the same sample along with RNA extraction?

I want to extract proteins and RNA from tissue samples. TRIzol reagent is not available in our labs. We have Qiazol instead? Does it do the same job? Are these products comparable in efficiency?...

14 October 2016 808 3 View

How can I purify extracted DNA from cell debris?

I extracted DNA using extraction column. It appeared that there was a lot of cell debris in the extract. Lot of impurities appeared in the nanodrop specking. How can I remove the cellular debris...

10 July 2015 905 7 View