What will be the appropriate antibody to evaluate cMYC expression in HELA cell line?

What will be the appropriate antibody to evaluate cMYC expression in HELA cell line?

11 December 2018 8,218 5 View

Please suggest appropriate cancer cell line for Farnnesyl Transferase Inhibitors?

Please suggest appropriate cancer cell line for Farnesyl Transferase Inhibitors

11 December 2018 6,992 0 View

Please suggest most appropriate PDB ID for MD simulations for cMYC G-Quadruplex stabilizing ligands?

Please suggest most appropriate PDB ID for MD simulations for cMYC G-Quadruplex stabilizing ligands?

11 December 2018 4,599 4 View

MD-Simulation tools for Windows?

MD-Simulation tools for Windows: Please suggest me free and efficient tool for simulation ligands with proteins and Nucleic acids sequences in Windows Interface?

11 December 2018 8,116 3 View

MD simulations for G-Quadruplex stabilizing agents?

Please suggest MD simulation software for windows interface.

10 November 2018 7,140 1 View

Thiazole orange displacement method for G-Quadruplex is valid way to evaluate compounds or not?

Dear All, Please share your experiences on thiazole displacement assay method to evaluate G-Quadruplex stabilzing agents?

02 March 2018 3,942 2 View

Guass view is not running in my windows system.Showing error that fail to establish directory file..Please share your experiences?

I did installation of guass view 03 in my windows based system but it is showing error of faile to establish temp directory. What may be the error please resolve my problem

31 December 2014 4,435 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View