Amended on 13 December 2018

I have searched cMYC/Myc proto-oncogene protein/Class E basic helix-loop-helix protein 39/Proto-oncogene c-Myc/Transcription factor p64 in my human protein database made from the PDMD (Protein-Direct-Microsequencing-Deciphering) method in vain (please see files; HepG2 Fucoidan and JMBT Alopecia). This may be due to famous species differences. PDMD method is a new protein identification and determination method without false-positive and false-negative.

Transcription factors (TFs) are also searched.

Human breast milk has TFs, but bovine milk has no TF.

Human breast milk has as follows; i.e., Zinc finger protein 610 at 19.6, Transmembrane and TPR repeat-containing porotein 1 at 7.7, DNA helicase V/Far upstream element-binding protein 1 at 4.9, Nuclear receptor corepressor 2 at 2.5, Transcription factor IIIA at 1.5, and Transcription regulator protein BACH2 at 0.7 μg/mg of milk protein.

Fetal hepatocyte Hc has as follows; i.e., Coiled-coil and C2 domain-containing protein 1B/Freud-2 at 2.9, Histone deacetylase 4/HDAC4 at 15.0, Modulator recognition factor 2/AT-rich interactive domain-containing protein 5B at 8.6, Nuclear protein ZAP3/YLP motif-containing protein 1 at 5.7, Poly [ADP-ribose] polymerase 12 /ADP-ribosyltransferase diphtheria toxin-like 12 at 2.2, Protein kinase C eta type at 42.5, Protein regulator of cytokinesis 1/PRC1 at 13.8, Protein SSX8 at 16.7, Transcription factor Sp5 at 7.3, and Zinc finger protein LOC400713/Zinc finger protein 880 at 5.5 μg/mg of cell protein. Total TFs becomes to be 89.7 μg/mg of cell protein (8.97% among cell proteins).

Actively growing cells seem to have many TFs.

Hepatoma HepG2 (cultured without fucoidan) has many TFs as follows.

Myb-related protein B at 5.3, Breast cancer anti-estrogen resistance 2/Transcriptional-regulating factor 1 at 4.8, Breast tumor novel factor 1/Chordin-like protein 2 at 0.97, Bromodomain and WD repeat domain-containing protein 1 at 8.0, Cold shock domain-containing protein E1/N-ras upstream gene protein at 4.5, CREB-binding protein at 1.8, E3 ubiquitin-protein ligase TRIM33/Transcription intermediary factor 1-gamma/Tripartite motif-containing protein 28 at 3.9, Transcription factor HIVEP2/HIV-EP2/Human immunodeficiency virus type I enhancer-binding protein 2/HIV-1-EP2 at 5.4, Hypoxia-inducible factor 1alpha at 1.4, Myelin transcription factor 1 at 3.9, Pinin at 0.76, REST corepressor 3 at 2.6, SH2 (Src homology 2) domain-containing protein 4A at 0.9, SWI/SNF complex subunit SMARCC1/SWI/SNF complex 155 kDa subunit at 3.1, TATA-binding protein-associated factor 172/ATP-dependent helicase BTAF1 at 11.8, Transcription factor Dp-1 at 0.22, Tumor protein p53-inducible nuclear protein 1/Stress-induced protein at 0.16, Unconventional prefoldin RPB5 interactor 1/RNA polymerase II subunit 5-mediating protein/Protein phosphatase 1 regulatory subunit 19 at 1.7, Zinc finger protein 16/Zinc finger protein KOX9 at 1.4, Zinc finger protein 366/Dendritic cell-specific transcript protein at 2.3, Zinc finger protein 614 at 3.1, Zinc finger protein 694/Zinc finger protein with KRAB and SCAN domains 2 at 1.7, Zinc finger protein 710 at 2.0, and Zinc finger protein 84/Zinc finger protein HPF2 at 4.7 μg/mg of cell protein, respectively. Total TFs becomes to be 76.41 μg/mg of cell protein (7.64% among cell proteins).

Healed hepatocyte HepG2 (cultured with fucoidan at 0.106 mg/mL for 3 days) has following TFs.

C-myc promoter-binding protein/DENN domain-containing protein 4A at 2.2, Zinc finger and SCAN domain-containing protein 2 at 0.27, Zinc finger MYM-type protein 1 at 4.1, Zinc finger protein 445 at 0.82, Zinc finger protein 460 at 0.72, and Zinc finger protein 518 at 6.3 μg/mg of cell protein, respectively. Total TFs becomes to be 14.41 μg/mg of cell protein (1.44% among cell proteins).

Therefore, the growth rate of fucoidan treated Hepatoma cells in 3 days has been reduced c.a. 5.3-fold (please see file again; HepG2 Fucoidan). The Fucoidan effect onto the cancer cells is due to reduction of the growth rate, and is not due to the notorious Apoptosis.

I am very gratefull to Dr. Vijay Nimbarte (Department of Chemistry and Biochemistry, Goethe-Universität Frankfurt am Main, Frankfurt am Main, Germany) for leading me to this important result.

By the way, Fucoidan can reduce the expression of TFs via reducing vira of HIV-1 and HCV. Fucoidan does not reduce the gene expression of TFs of fetal normal Hc cells. Expression of TFs in fetal cells is not induced by virus. TFs expression in fetal cells seems to be induced by cells' intrinsic developing power. Therefore, fucoidan is the safe drug without side effect to normal cells (please see file; Rat DEN Np-Fuco).

Kou Hayakawa Thanks

Further added on 11 December 2018

Since gene expression of TFs seems to be important for me, I have further studied the gene expression of TFs.

Human breast milk has expressed TFs at 36.9 μg/mg of milk protein. This milk has up regulating virus of Protein Bel-2 (Macaque simian foamy virus/SFV) at 21.9 μg/mg of milk protein. Therefore, mammary gland synthesize TFs at 40% by the expression power of the organ, and at 60% by the power of virus.

Fetal hepatocyte Hc has expressed TFs at 120.2 μg/mg of cell protein. This cell has up regulating virus of Biotin synthetase (Bacillus subtilis) at 2.7, Env polyprotein/Envelope glycoprotein gp160 (HIV type 2/HIV-2) at 3.1, Genome polyprotein (Dengue virus 92/DENV) at 6.9, Genome polyprotein (ECHOvirus 9/Enteric Cytopathic Human Orphan virus) at 15.3, and Genome polyprotein (HCV) at 2.3 μg/mg of cell protein, respectively. Repressing bacterium of Rickettsia is not present in this cell. Then, total inducing power becomes to be 30.3 μg/mg of cell protein. Therefore, fetal developing cells seem to produce 75% of TFs by their own power, and 25% is due to bacterium and virus.

Healed hepatocyte HepG2 (treated by edible Fucoidan at 0.102 mg/mL for 3 days) has expressed TFs at 14.41 μg/mg of cell protein. This healed normal cell has up regulating virus of Biotin synthetase (Bacillus subtilis) at 4.8, Genome polyprotein (HCV) at 1.9, Non-structural protein 4b (Human coronavirus 229E/HCoV-229E) at 0.2, and Protein Nef (HIV-1) at 0.8 μg/mg of cell protein, respectively. Repressing bacterium of Rickettsia is not present in this cell. Then, total inducing power becomes to be 7.70 μg/mg of cell protein. Therefore, healed normal cells seem to produce 45% of TFs by their own power, and 53% is due to bacterium and virus.

Hepatoma HepG2 (cultured without edible Fucoidan for 3 days) has expressed TFs at 71.11 μg/mg of cell protein. This hepatoma cell has up regulating virus of Biotin synthetase (Bacillus subtilis) at 1.4, Envelope glycoprotein gp160 (HIV-1) at 15.8, Nef/Negative factor (HIV-2) at 0.61, Envelope glycoprotein gp95 (Rous sarcoma virus/RSV) at 0.5, Genome polyprotein (HCV) at 7.8, Polyprotein (GB virus C/GBV-HGV/GBV-C) at 15.4, and Genome polyprotein (Human poliovirus/PV) at 5.1 μg/mg of cell protein, respectively. Repressing bacterium of Rickettsia is not present in this cell. Then, total inducing power becomes to be 46.61 μg/mg of cell protein. Therefore, hepatoma cells seem to produce 34% of TFs by their own power, and 66% is due to virus and bacterium.

It is interesting that edible Fucoidan has dramatically reduced the expression power of TFs by 16.5% or 6.05-fold in 3 days.

Amended on 13 December 2018

The expression of TFs in diseased human livers and non-cancer human serum has also intrigued us.

Diseased human livers;

Liver tissue (with pseudo liver cancer; has been survived) has TFs of AT-rich interactive domain-containing protein 1A/SWI/SNF complex protein P270/B120/ARID domain-containing protein 1A at 9.8, Bone morphogenetic protein 7/Osteogenic protein 1 at 21.3, cAMP-response element binding protein/CreB at 1.5, Cyclic AMP-dependent transcription factor ATF-1/Protein TREB36/Activating transcription factor 1 at 1.5, DNA-directed RNA polymerase II subunit RPB1/DNA-directed RNA polymeras II subunit 2 at 4.8, HIV-EP2/Transcription factor HIVEP2 at 16.3, Homeobox protein Hox-B7/Homeobox protein Hox-2C at 1.3, Forkhead box protein N2/Human T-cell leukemia virus enhancer factor/HTLF at 1.3, Insulinoma-associated protein 1/Zinc finger protein IA-1 at 0.44, Mitogen-activated protein kinase kinase kinase 9/Mixed lineage kinase 1/MAP3K9 at 14.9, Serine/threonine-protein kinase receptor R5/ALK-3/Bone morphogenetic protein receptor type-1A at 1.7, Transcription factor AP-4/Class C basic helix-loop-helix protein 41 at 2.0, Zinc finger E-box-binding homeobox 1/NIL-2-A zinc finger protein at 1.6, and Zinc finger Y-chromosomal protein at 7.3 μg/mg of tissue protein, respectively. Total TFs become to be 85.74 (8.57%). The prerequisite for survival seems to be the expression of TFs less than 11%. This relatively high TFs (8.57%) in this non-cancer liver may have lead to the surgeons to diagnose as "psedo liver cancer" after the Abdominal surgery celiotomy or Laparotomy.

This liver has up regulating virus of Envelope glycoprotein gp160 (HIV-2) at 8.2, Gag-Pol polyprotein (HIV-1) at 3.9, Gag-Pro-Pol polyprotein (BoLV/Bovine leukemia virus) at 4.3, AKT kinase-transforming protein/v-AKT (AKT8 Murine leukemia virus/AKT8 MuLV) at 2.0, Genome polyprotein (HCV) at 4.4, Genome polyprotein (Dengue virus type-4; DENV) at 5.2, Spike glycoprotein (Human coronavirus/HCoV) at 14.8, ORF1a (Avian infectious bronchitis virus/IBV/ACoV) at 46.0, and RNA-directed RNA polymerase (Murine hepatitis virus/MCoV) at 3.2 μg/mg of tissue protein, respectively. Repressing bacterium of Rickettsia is present (DNA gyrase subunit B (Rickettsia conorii)) at 2.0 μg/mg of tissue protein. Then, total inducing power becomes to be 89.0 (8.90%) μg/mg of tissue protein. It is interesting that the expression of TFs in old person seems to be totally (100%) depended upon the power of virus.　　　　　　

LC tissue (with leprosy, female; has been sadly died) has Acute myeloid leukemia 1 protein/CBF-alpha-2/Runt-related transcription factor 1/Oncogene AML-1 at 0.27, Beta-Catenin/Catenin beta-1 at 3.2, COUP transcription factor/COUP-TF at 2.4, GTPase KRas at 0.33, Homeobox protein OTX1 at 6.6, Homeobox/POU domain protein RDC-1/Brain-specific homeobox/POU domain protein 3A at 1.6, Interferon-induced, double-stranded RNA-activated protein kinase/P68 kinase at 3.6, Mineralocorticoid receptor at 0.19, Nuclear autoantigen Sp-100/Speckled 100 kDa at 0.86, Ring3 protein/Really interesting new gene 3 protein/Bromodomain-containing protein 2 at 3.1, Serine/threonine-protein kinase A-raf/A-RAF proto-oncogene serine/threonine-protein kinase at 3.7, Serine/threonine-protein kinase B-raf/B-Raf proto-oncogene serine/threonine-protein kinase/P94 at 1.4, TGF-beta receptor type-1/Serine/threonine-protein kinase receptor R4/ALK-5 at 4.4, Transcription factor jun-D at 1.6, Transcription initiation factor IIF subunit alpha/General transcription factor IIF subunit 1/Transcrition factor IIF, alpha subunit at 6.5, Transcription initiation factor IIF subunit alpha/Transcrition factor IIF, alpha subunit at 3.4, Transducin-like enhancer protein 1/Enhancer of split groucho-like protein 1 at 5.0, and Zinc finger protein HRX/Histone-lysine N-methyltransferase 2A at 72.5 μg/mg of tissue protein, respectively. Total TFs become to be 120.65 (12.07%) μg/mg of tissue protein. It is intersting that even LC tissue has high TFs, and she has sadly deceased. This high TFs may be partly related to her leprosy.

This LC liver tissue has up regulating virus of Genome polyprotein (HCV) at 21.9, Genome polyprotein (HAV) at 14.4, v-Mil (v-Raf) serine/threonine-protein kinase (Avian rous-associated virus/RAV) at 2.0, Nucleocapsid protein (Feline infectious peritonitis virus/FCoV) at 3.4, L protein (Murine respirovirus/Sendai virus/SeV/HVJ) at 13.3, RNA replicase polyprotein (Turnip yellow mosaic virus/TYMV) at 16.2, Genome polyprotein (Bean yellow mosaic virus/BYMV) at 1.5, Beta-Glucosidase/Gentiobiase (Agrobacterium tumefaciens) at 0.29, Wide host range VirA protein/WHR VirA (Ti bacteriophage of Agrobacterium tumefaciens) at 8.1 μg/mg of tissue protein, respectively. Repressing bacterium of Rickettsia is absent. Then, total inducing power becomes to be 81.09 (8.11%) μg/mg of tissue protein. TFs of this leprosy LC liver is up regulated by 67%of virus and bacteria, and by 33% from her own power.

HCC tissue (with PBC, old female; has been sadly deceased) has Androgen receptor/Nuclear receptor subfamily 3, group C, member 4 at 7.1, A-Raf proto-oncogene serine/threonine-protein kinase/Serine/threonine-protein kinase A-Raf/Oncogene PKS2 at 1.8, CCAAT/enhancer-binding protein beta/Nuclear factor NF-IL6/C/EBP beta at 3.6, DNA-directed RNA polymerase II subunit RPB1/Homeobox protein MSX-1 at 33.5, Homeobox protein MSX-1/Hox-7 at 1.1, Mast/stem cell growth factor receptor Kit at 3.0, Tyrosine-protein kinase ABL1/Proto-oncogene c-Abl/Abelson murine leukemia viral oncogene homolog 1 at 7.2, Receptor tyrosine-protein kinase erbB-2/Erb b-2 receptor protein-tyrosine kinase/Proto-oncogene Neu at 5.7, Ring3 protein/Really interesting new gene 3 protein/Bromodomain-containing protein 2 at 7.7, Steroid hormone receptor ERR2 at 2.2, Transcriptional activator GLI3/Gli3 protein at 11.7, Transcription initiation factor TFIID subunit 1/TAFII-250 at 20.3, and Voltage-dependent L-type calcium channel subunit beta-1/Calcium channel beta-1 subunit, dihydropyridine-sensitive L-type/CACNB1 at 19.1 μg/mg of tissue protein, respectively. Total TFs become to be 124.0 (12.40%) μg/mg of tissue protein.

This HCC tissue has up regulating virus of Gag polyprotein (Visna Maedi virus/Ovine lentivirus/Maedi-Visna virus/VMV) at 8.9, Virion infectivity factor (HIV-1) at 12.9, Gag-Pol polyprotein (HIV-2) at 3.4, Gag-Pol polyprotein (SIV) at 8.6, Genome polyprotein (Hog cholera virus/Classical swine fever virus/CSFV) at 43.6, Genome polyprotein (HAV) at 24.0,and Genome polyprotein (HCV) at 35.4 μg/mg of tissue protein, respectively. Repressing bacterium of Rickettsia is absent. Then, total inducing power becomes to be 136.8 (13.7%) μg/mg of tissue protein. TFs of this HCC tissue is up regulated totally (100%) depending upon the power of virus.

LC tissue (named as No.6, male; this patient has survived) has ENL protein/YEATS domain-containing protein 1 at 1.7, Gli3 protein/Transcriptional activator GLI3 at 30.2, Helix-loop-helix protein 2/HEN2 at 1.4, Hepatocyte nuclear factor 1-beta/HNF-1B at 1.2, High mobility group protein B1/HMG-1/High mobility group protein 1 at 0.21, HIV-EP2 at 13.8, Homeobox protein HOX-B7/Homeobox protein Hox-2C at 0.6, Polycomb group RING finger protein 2/DNA-binding protein MEL-18 at 0.36, Prohibitin at 0.7, Ras-like protein TC10/Rho-related GTP-binding protein RhoQ/Ras-like protein family member 7A at 0.23, Serine/threonine protein kinase receptor R5/Bone morphogenetic protein receptor type-1A/ALK-3 at 2.2, SET protein/SET nuclear proto-oncogene at 2.6, Sterol regulatory element binding protein 1/Class D basic helix-loop-helix protein 1/SREBP-1 at 42.8, Wilms' tumor protein/WT33 at 1.8, and Zinc finger protein 90/Zinc finger protein HTF9 at 0.53 μg/mg of tissue protein, respectively. Total TFs become to be 100.33 (10.0%) μg/mg of tissue protein.

This LC tissue has up regulating virus of Virion infectivity factor (feline immunodeficiency virus/FIV) at 0.42, VPX protein (HIV-2) at 1.3, Gag polyprotein (Rous sarcoma virus/RSV) at 18.7, Pol polyprotein (SIV) at 7.5, RNA-directed RNA polymerase (Murine coronavirus/MCoV) at 29.1, Genome polyprotein (HCV) at 7.9, Genome polyprotein (Louping ill virus/LIV) at 6.4, Genome polyprotein (Mosquit cell fusing agent) at 48.9, and Genome polyprotein (Dengue virus/DENV-4) at 0.31μg/mg of tissue protein, respectively. Repressing bacterium of Rickettsia is absent. Then, total inducing power becomes to be 94.93 (9.49%) μg/mg of tissue protein. TFs of this LC tissue is up regulated at 95% depending upon the power of virus. 　　　

HCC tissue (No.6) has B-cell lymphoma 3 protein/BCL-3 protein/Proto-oncogene BCL3 at 1.2, Blym-1 protein at 0.82, Bone morphogenetic protein 2 at 8.9, Breast cancer type 1 susceptibility protein/RING finger protein 53/RING-type E3 ubiquitin transferase BRCA1 at 7.4, Cellular tumor antigen p53 /Tumor suppressor p53 at 0.22, DNA-(Apurinic or apyrimidinic site) lyase at 0.21, DNA-repair protein complementing XP-G cells at 24.2, E3 ubiquitin-protein ligase CBL/Proto-oncogene c-CBL at 0.055, HIV-EP2 at 9.5, Norrin/Norrie disease protein/X-linked exudative vitreoretinopathy 2 protein at 0.011, Possible global transcription activator SNF2L/ATP-dependent helicase SMARCA1 at 5.2, Ring3 protein/Really interesting new gene 3 protein at 5.2, Serine/threonine-protein kinase A-Raf/Proto oncogene Pks2 at 0.53, and Transcription factor-7/T-cell-specific transcription factor 1/TCF-1 at 3.2 μg/mg of tissue protein, respectively. Total TFs become to be 61.446 (6.14%) μg/mg of tissue protein. It is interesting that this HCC portion is lower in TF expression than LC portion.

This HCC tissue has up regulating virus of TAT protein (SIV) at 15.8, RNA-directed RNA polymerase (Human coronavirus/HCoV) at 10.4, X2A protein (Porcine transmissible gastroenteric coronavirus strain Purdue/TGEV/PCoV) at 0.09, ORF1a (Avian infectious bronchitis virus/IBV/ACoV) at 10.9, Matrix protein (Murine respirovirus/Sendai virus/SeV) at 4.9, Genome polyprotein (Dengue virus/DENV-4) at 19.8, Genome polyprotein (HCV) at 0.61, and Genome polyprotein (Hog cholera virus/Classical swine fever virus/CSFV) at 0.74 μg/mg of tissue protein, respectively. Repressing bacterium of Rickettsia is absent. Then, total inducing power becomes to be 63.24 (6.32%) μg/mg of tissue protein. TFs of this HCC tissue is up regulated totally (100%) by the power of virus. 　　　　　　　　

Further added on 13 December 2018

Non-cancer human serum;

Serum of GSD-1b patient (3y girl, biotin deficiency; with alopecia) has ATP-dependent helicase CHD1/Chromodomain-helicase-DNA-binding protein 1at 9.1, DNA damage-binding protein 1/HBV X-associated protein 1/AH receptor-interacting protein/Aryl-hydrocarbon receptor-interacting protein at 1.7, Histone-lysine N-methyltransferase MLL3/Lysine N-methyltransferase 2C at 9.3, RNA polymerase-associated protein CTR9 homolog/SH2 domain-binding protein at 7.0, and Zinc finger protein 441 at 6.2 μg/mg of serum protein. Total TFs become to be 33.3 (3.33%) μg/mg of serum protein.

This serum has up regulating virus and bacteria of DNA-directed RNA polymerase subunit beta (Bacillus anthracis) at 5.9, Uncharacterized transporter ycbK (Bacillus subtilis) at 8.2, Protein Tat/Transactivating regulatory protein (Simian IV/SIV) at 7.6, Replicase polyprotein 1a (Murine hepatitis virus/MHV/Murine coronavirus/MCoV) at 11.0, Cytadherence accesory protein 2 (Mycoplasma pneumonia) at 1.6, and Oligopeptidase A (Haemophilus influenzae) at 6.1 μg/mg of serum protein, respectively. Repressing bacterium of Rickettsia is absent. Then, total inducing power becomes to be 40.4 (4.04%) μg/mg of serum protein. This 100% induction of TFs by Virus and Bacteria is not children's character at all, and this may be related her biotin deficiency.

The serum of the above patient (alopecia has been cured by biotin therapy, after 2mo of liver transplantation) has TFs of Epidermal growth factor receptor/EGFR/Proto-oncogene c-ErbB-1/Receptor tyrosine-protein kinase erbB-1at 3.6, Mediator complex subunit 14/Mediator of RNA polymerase II transcription subunit 14 at 3.5, Paternally-expressed gene 3 protein at 4.0, Renal carcinoma antigen NY-REN-36/Zinc finger protein 608 at 4.1, Transcription initiation factor TFIID subunit 3 at 2.7, and Zinc finger protein 800 at 9.9 μg/mg of serum protein. Total TFs become to be 27.8 (2.78%) μg/mg of serum protein.

This serum has up regulating virus and bacteria of Spore protease (Bacillus cereus) at 3.9, and Genome polyprotein (HCV) at 4.0 μg/mg of serum protein, respectively. Then, total inducing power becomes to be 7.9 (0.79%) μg/mg of serum protein. This serum has down regulating bacteria of Putative uncharacterized protein RP311 (Rickettsia prowazekii) at 4.5, and Isopentenyl pyrophosphate isomerase (Rickettsia prowazekii) at 3.2 μg/mg of serum protein, respectively. Then, total inducing power becomes to be (7.9　－　7.7　＝) 0.2 μg/mg of serum protein or 0.02%. This patient has synthesized 100% by her own force. Biotin therapy or liver transplantation may have effected on this phenomenon.

Serum of 4mo boy (with biotin deficiency and alopecia) has Geminin at 3.3, Histone acetyltransferase MYST3/Histone acetyltransferase KAT6A at 11.3, Histone-lysine N-methyltransferase 2B/Trithorax homolog 2 at 4.5, PDH finger protein 12 isoform 1 at 3.6, Protein bicaudal C homolog 1 at 5.4, S phase cyclin A-associated protein in the ER/Zinc finger protein 291 at 12.6, Transcriptional regulator Kaiso/Zinc finger and BTB domain-containing protein 33 at 2.6, and Zinc finger protein 143 at187 at 1.5 μg/mg of serum protein, respectively. Total TFs become to be 44.9 (4.49%) μg/mg of serum protein.

This serum has up regulating virus and bacteria of Replicase polyprotein 1ab (Feline infectious peritonitis virus/FCoV) at 3.6, Succinate dehydrogenase flavoprotein subunit /SDH (Bacillus subtilis) at 14.8, and Probable cysteine desulfurase (Mycoplasma genitalium) at 23.2 μg/mg of serum protein, respectively. Then, total inducing power becomes to be 41.6 (4.16%) μg/mg of serum protein. This serum has down regulating bacteria of DNA primase (Rickettsia typhi) at 3.6 μg/mg of serum protein. Then, total inducing power becomes to be (41.6　－　3.6　＝) 38.0 μg/mg of serum protein or 3.08%. This patient's inducing power is depended on 85% on Bacteria and Virus. This 85% induction of TFs by Virus and Bacteria is not children's character at all, and this may be related her biotin deficiency.

The serum of the above patient (alopecia has been cured by biotin therapy for 13w) has TFs of Breast cancer-associated antigen BRCAA1/ARID domain-containing protein 4B/AT-rich interactive domain-containing protein 4B at 4.1, E3 ubiquitin-protein ligase RAG1/V(D)J recombination-activating protein 1 at 4.0, GDNF(glial cell line derived neurotrophic factor)-inducible zinc finger protein 1 at 1.8, and Metallothionein-1F at 11.3 μg/mg of serum protein, respectively. Total TFs become to be 21.2 (2.12%) μg/mg of serum protein.

This serum has up regulating Virus and Bacteria of Virion infectivity factor/Vif (HIV-1) at 3.7, Gag polyprotein (Koala retrovirus/KoRV) at 2.2, and Aspartyl-tRNA synthetase (Haemophilus influenzae) at 21.3 μg/mg of serum protein, respectively. Then, total inducing power becomes to be 32.8 (3.28%) μg/mg of serum protein. This serum has down regulating bacteria of R1pA-like lipoprotein (Richettsia felis) at 8.5 μg/mg of serum protein. Then, total inducing power becomes to be (32.8　－　8.5　＝) 24.3 μg/mg of serum protein or 2.43%. This patient's inducing power is still depended on 100% on Bacteria and Virus. This 100% induction of TFs by Virus and Bacteria is not children's character at all, and this patient may have not been cured perfectly within 13w.

Serum of 1y girl (with biotin deficiency and Alopecia) has DNA (cytosine-5)-methyltransferase 3A/DNA methyltransferase HsaIIIA at 2.1, Guanine nucleotide exchange factor DBS at 19.4, Myocardin at 2.7, Nucleolar complex protein 2 homolog at 4.5, Protein arginine N-methyltransferase 7 at 6.8, Retinoblastoma-binding protein 1-like 1 at 4.7, Scm-like with four MBT domains protein 2 at 12.0, and Zinc finger protein 674 at 2.0 μg/mg of serum protein, respectively. Total TFs become to be 54.2 (5.42%) μg/mg of serum protein.

This serum has up regulating Bacteria of Probable dethiobiotin synthetase 1 (Haemophilus influenzae) at 12.7, and repressing bacteria of Repress Uncharacterized lipoprotein RT0028 (Rickettsia typhi) at 5.1 μg/mg of serum protein, respectively. Then, total inducing power becomes to be (12.7　－　5.1　＝) 7.6 μg/mg of serum protein or 0.76%. This patient is expressing at 85％ by her inducing power, and her biotin deficiency seems to be not caused by Bacterial infection.

The serum of the above patient (alopecia has been cured by biotin therapy for 16w) has TFs of Abl interactor 2/Abelson interactor 2/Abi-2 at 9.5, Fetal liver LKB1-interacting protein/TNFAIP3-interacting protein 2 at 11.3, Growth differentiation factor 8/GDF8/Myostatin at 11.9, T-box transcription factor TBX3 at 4.0, Transcription factor HIVEP3 at 7.1, Transcription initiation factor TFIID subunit 3 at 3.0, Zinc finger MYM-type protein 4/Zinc finger protein 262 at 7.7, and Zinc finger protein 263 at 4.7 μg/mg of serum protein, respectively. Total TFs become to be 59.2 (5.92%) μg/mg of serum protein.

This serum has no regulating Bacteria at all, and she has made TFs by her own power. Biotin therapy for 16w seems to be perfectly effected.

The serum of the above patient (alopecia has been cured by biotin therapy for 16w, but biotin excess has been occurred at 44w) has TFs of Coiled-coil domain-containing protein 138/CCDC138 at 10.2, Homeobox protein engrailed-2/Homeobox protein en-2 at 3.1, Oligodendrocyte transcription factor 3 at 4.3, Ras-interacting protein 1 at 4.0, RNA polymerase-associated protein CTR9 homolog at 7.8, Vascular endothelial zinc finger 1 at 2.1, V-maf musculoaponeurotic fibrosarcoma oncogene homolog B/Transcription factor Maf at 2.9 μg/mg of serum protein, respectively. Total TFs become to be 30.1 (3.01%) μg/mg of serum protein.

This serum has up regulating Virus and Bacteria of Genome polyprotein (Human hepatitis A virus/HAV) at 10.7, Genome polyprotein (HCV) at 7.6, Immunoglobulin A1 protease autotransporter (Haemophilus influenzae) at 8.4, Pol polyprotein (AKR (endogenous) murine leukemia virus/AKV Murine leukemia virus) at 3.4 ,Replicase polyprotein 1a (Human coronavirus OC/HCoV OC) at 10.5, γ-D-Glutamate-meso-diaminopimelate muropeptidase lytF (Bacillus subtilis) at 6.2 μg/mg of serum protein, respectively. Total inducing power is 69.1 (6.91%). Repressing power is DNA ligase (Rickettsia prowazekii) at 20.0, and Phenylalanine-tRNA ligase beta chain (Rickettsia typhi) at 4.2 μg/mg of serum protein, respectively. Then, total inducing power becomes to be (69.1　－　24.2 =) 44.9 (4.49%). This patient has been returned to be disease, (excess biotin), since her production of TFs is 100% depended on Virus and Bacteria.

Serum of 8mo girl (with Gastritis) has Atrophin-1-like protein/Arginine-glutamic acid dipeptide repeats protein at 9.4, DNA helicase B at 9.8, Forkhead box (FOX) protein D3/HNF3/FH transcription factor genesis at 7.0, PH (Pleckstrin homology)-interacting protein/IRS-1 PH domain-binding protein at 17.5, Transcription elongation factor A protein-like 5/TCEA-like protein 5 at 6.1, Zinc finger protein 37 homolog at 4.9, Zinc finger protein 499/Zinc finger and BTB domain-containing protein 45 at 6.0, Zinc finger protein 746/Parkin-interacting substrate at 1.7 μg/mg of serum protein, respectively. Total TFs becomes to be 62.4 (6.24%).

This serum has inducing power of Replicase polyprotein 1ab (Human coronavirus 229E/HCoV 229E) at 17.7, and Phenylalanyl-tRNA ligase beta chain (Mycoplasma hyopneumoniae) at 3.1 μg/mg of serum protein, respectively. Total inducing power is 20.8 μg/mg of serum protein. Repressing power is present at Uncharacterized protein RP821 (Rickettsia prowazekii) 8.0 μg/mg of serum protein. Then, total inducing power becomes to be 12.8 μg/mg of serum protein. This girl has expressed 81％ by her own power, and she is now growing and developing.

Serum of 12mo girl as above (with common cold) has Agrin at 16.7, Gastric cancer antigen Ga19/N-alpha-acetyltransferase 15/NatA at 13.0, Peroxisome proliferator-activated receptor alpha at 13.5, Serine/threonine-protein kinase Chk2 at 11.4 μg/mg of serum protein, respectively. Total TFs becomes to be 54.6 (5.46%) μg/mg of serum protein.

This serum has inducing power of Replicase polyprotein 1ab (Avian infectious bronchitis virus/IBV/ACoV) at 9.4, and Conserved hypothetical protein/UPF247 protein MYPU_2540 (Mycoplasma pulmonis) at 12.2 μg/mg of serum protein, respectively. Reducing Rickettsia is not present. Then, total inducing power becomes to be 21.6 (2.16%) μg/mg of serum protein. She has produced TFs at 60％ by her own power, and age of 12mo is still growing and developing.

Adult serum;

Serum of Gait disorder patient (32y, female) has Epidermal growth factor receptor/Proto-oncogene c-ErbB-1 at 1.5, Ovarian zinc finger protein/NF-X1-type zinc finger protein NFXL1 at 1.8, Uncharacterized protein C12orf12/Coiled-coil domain-containing glutamate-rich protein 1 at 7.3, Zinc finger protein 432 at 2.0 μg/mg of serum protein, respectively. Total TFs becomes to be 12.6 (1.26%) μg/mg of serum protein. TF amount seems to be low, this may be due to her diseases (Gait disorder, Narrowing of the visual field).

This serum has inducing power of 6-Phospho-beta-glucosidase (Bacillus subtilis) at 3.7, S glycoprotein (Canine coronavirus/CCoV) at 5.9, Type III restriction-modification system Bce10987IP enzyme res (Bacillus cereus) at 5.3, Genome polyprotein (HCV) at 5.3 μg/mg of serum protein, respectively. Total inducing power is 20.2 μg/mg of serum protein. This serum has reducing power of DNA gyrase subunit A (Rickettsia felis) at 4.0 μg/mg of serum protein. Total inducing power is 16.2 （1.62%）μg/mg of serum protein. 100% of inducing power is from Virus and Bacteria, and this seems to be due to her disease.

Serum of sister (22y) of above patient has PHD finger protein 20/Hepatocellular carcinoma-associated antigen 58/Glioma-expressed antigen 2 at 7.4, Nuclear receptor subfamily 6 group A member 1/Germ cell nuclear factor at 4.6, Nucleotide-binding oligomerization domain-protein 14 at 7.1, Proto-oncogene TRE-2/Ubiquitin carboxyl-terminal hydrolase 6/USP6 at 2.8, Zinc finger protein 787 at 11.6 μg/mg of serum protein, respectively. Total TFs is 33.5 (3.35%) μg/mg of serum protein.

This serum has up-regulate power of Oligopeptide transport ATP-binding protein oppF (Mycoplasma pneumoniae) at 2.9 μg/mg of serum protein.

Repress power is Aspartyl-tRNA synthetase (Richettsia prowazekii) at 2.9, and Putative ankyrin repeat protein RBE_0601 (Rickettsia bellii) at 12.5 μg/mg of serum protein, respectively.

This adult still resembles to be child, since she produces TFs by her own power.

Serum of mother (52y) of above patient has Mediator of RNA polymerase II transcription subunit 13/Vitamin D3 receptor-interacting protein complex component DRIP250 at 9.2, Ovarian cancer-related protein 10-2/Teashirt homolog 2/Zinc finger protein 218 at 3.1, Peroxisome proliferator-activated receptor gamma coactivator-related protein 1/PRC/PGC-1-related coactivator at 19.5, PHD finger protein 1/Polycomb-like protein 1 at 4.2, Putative zinc finger protein 840 at 2.2, SEC14-like protein 2/Tocopherol-associated protein 2/ Squalene transfer protein at 10.4, Zinc finger homeobox protein 4 at 5.0, Zinc finger protein 831 at 9.8, and Zinc finger protein 569 at 4.0 μg/mg of serum protein, respectively. Total TFs is 67.4 (6.74%) μg/mg of serum protein.

This serum has inducing power of Replicase polyprotein 1a (Human coronavirus 229E/HCoV 229E) at 0.3, ＣysZ (Haemophilus influenzae) at 21.4, Non-structural protein NS2 (Bluetongue virus/BTV) at 18.4, Ribonucleoside-diphosphate reductase subunit beta (Mycoplasma pneumoniae) at 22.1 μg/mg of serum protein, respectively. Total inducing power is 72.2 μg/mg of serum protein.

Repress power is UDP-glucose 6-dehydrogenase (Rickettsia prowazekii) at 7.9 μg/mg of serum protein. Therefore, total inducing power becomes to be 64.3 (6.43%) μg/mg of serum protein. Since inducing of TFs is completely under the control of Bacteria and Virus, 52y seems to be complete adult.

Serum of healthy man (33y) has ATP-dependent chromatin remodelling protein/Bromodomain adjacent to zinc finger domain protein 1A at 6.6, Histone acetyltransferase KAT7/MYST2 at 7.7, Host cell factor 2 at 6.9, Nuclear receptor subfamily 4 group A member 2/Orphan nuclear receptor NURR1 at 2.8, Protein PML at 5.0, and Protein Wnt5a at 9.1, Zinc finger protein 185 at 6.9 μg/mg of serum protein, respectively. Total TFs is present at 38.1 (3.81%) μg/mg of serum protein.This serum has no inducing and/or repressing Bacteria and Virus.

Since inducing of TFs is completely under the control of his own power, 33y seems to be not yet the complete adult.