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Questions related from Laurence Stuart Dawkins-Hall
Dear Colleagues I have a colleague who is not expecting an antibody in her lysate to give expression of a protein at about 23kD (owing to gene methylation) However, the antibody does give bands...
12 December 2019 3,726 3 View
Dear Colleague(s) this is really an extension of my previous question relating to Western blotting on Behalf of the same colleague How valid would you consider a Western blot that does indeed...
12 December 2019 8,741 4 View
Dear User 1. I am currently part of a research project where total RNA is extracted from tiny fractions of patient clinical samples. Consequently, we are getting 50ng to 100ng of total RNA: 2. We...
12 December 2017 975 3 View
Dear scientists' has anybody used Lonza pre cast TBE acrylamide gels and if so what are your experiences compared to popular alternatives like Invitrogen Novex and Biorad gels ?
02 February 2017 6,597 4 View
Dear Colleague(s) I am about to clone a 200bp PCR fragment with restriction fragment overhangs Its been quite a few years since I performed this routine technique so I am wondering what are the...
10 October 2016 374 7 View
Dear Colleague(s) I am dealing with a gene at the moment that exhibits very low expression and is barely detectable from neat cDNA. As such if you dilute the cDNA to construct a standard curve...
06 June 2016 5,243 8 View
Dear Researcher I am trying to quantify a gene in 2 sample sets one of which yields a consistent discernible Ct value, albeit at the low end of meaningful detection (>35cycles) and one of which...
03 March 2016 1,450 3 View
Dear Colleague(s) I am trying to run REST 2009 on a PC platform with windows 7 OS; It doesn't appear to want to run Are there some compatibility issues ? Am I attempting to install and run the...
03 March 2016 5,159 6 View
Dear Colleague(s) I am well versed with real time qPCR but have never actually worked with human heart tissue to determine relative expression of a GOI, viz cytochrome p450 2J2 variant. In...
01 January 2016 3,838 2 View
Hi, I routinely measure gene expression by qPCR using the delta delta ct method, wherein a test gene is measured in treated and untreated samples and both are normalised (for loading/amplification...
10 October 2014 2,958 7 View
Dear Colleague(s) I am not a histone biologist but have been asked to contemplate the feasibility - indeed sensibility - of a histone binding assay: In essence we are proposing to look at the...
01 January 1970 4,995 4 View
