I have extracted surface proteins from a sample in 2x and 4x DPBS. While preparing standards (BSA) for quantification shall I use the diluent 2x/4x DPBS or mili Q water?
Carlo Zambonelli
Always use your buffer, although in this case H2O or DPBS will not give much of a difference.
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Rosa María Martínez-Espinosa
Dear Vipul:
Some reagents such as DPBS or detergents (Triton for instance) interfere with Bradbord assay, so I suggest you to prepare the standards using the same concentration of DPBS you have in your surface proteins sample.
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Samira Feyzi
Dear Vipul,
Sure, you should use the same buffer. Otherwise you will not be able to remove the interference effects of buffer on your reagents or ...
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Vipul Batra
Thank you all.
@ Rosa; If it interferes then why should I continue with DPBS?
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