Samira Feyzi

3 Questions 12 Answers 0 Followers

Questions related from Samira Feyzi

Do we have a hyperchromic shift in protein absorbance uv-vis spectrum when there is a red shift?

Changing the polarity of the protein environment causes shift in the wave length of maximum absorbance. ie. less polar solvent causes red shift. This is usually along with a shift in molar...

04 April 2016 5,980 7 View

How could we prove the data are related to unsteady state?

In a range of initial substrate concentrations at different reaction time of enzyme reaction plotting Michaelis menten equations and other linear equations including Linweaver-Burk, Woolf-Hans and...

07 July 2015 1,417 8 View

What is the effect of reducing agents like 2-Mercaptoethanol on protein bands and disulfide bands in SDS-PAGE pattern?

We have worked on the SDS-PAGE pattern of the protein isolate in 2 reduced and non-reduced conditions. In reduced condition with 2-mercapttoethanol, bands with higher molecular weight were...

07 July 2014 9,366 6 View