Surface extraction of proteins

More Vipul Batra's questions See All

Why the area measure is ZERO while using multi-point tool in ImageJ?

I am trying to count the number of sperm cells in a fluorescent labelled image. After using the multi-point tool when I click measure the area column for all the selected cells is ZERO. I need...

11 December 2018 8,392 3 View

What should be the sample in selection pressure analysis ?

While performing Phylogenetic and evolutionary analysis of a gene family what should be the sample ? I am working on a gene family that has undergone various duplication & speciation events...

08 September 2018 6,859 3 View

What is the difference between 2(-ddct) and fold change and relative expression?

I have calculated 2 (-ddct) for a set of 6 genes in 5 tissues using GAPDH as reference and one of the tissues as the calibrator. However, after computing the 2 (-ddct) values, which I believe is...

07 August 2018 1,622 1 View

How can I choose an appropriate Housekeeper if there is a huge intra sample variation in Ct values?

If there is a large intra sample variation amongst the housekeeping genes, how can I decide the best housekeeper. The samples are from a abattoir which show large variations in Cq values in the...

05 June 2018 7,390 4 View

Why do I get a large number of proteins post PI-PLC treatment of sperm?

I have treated 100 million spermatozoa with PI-PLC (2U/ml) in sp-TALP medium.The enzyme has specificity for GPI anchor. However, post MS analysis by TPP, approx 300 proteins are identified many...

02 March 2018 8,952 1 View

RNA integrity ?

Does any of the RNAs look good? I want to proceed for RACE. It is a 1.2% TAE gel. Not a denaturing gel. lane 1 is ds DNA ladder (1 Kb). Although I agree it should have been run for some more time....

05 June 2017 6,611 4 View

How can this graph be plotted for analysis of gene expression pattern?

I want to plot the results of my qPCR in a way similar to shown in the picture since there is lot of inherent biological variation. Could anyone please let me know which tool/program was used to...

04 May 2017 5,308 3 View

What will be the blank in bradford's assay?

I have extracted surface proteins from a sample in 2x and 4x DPBS. While preparing standards (BSA) for quantification shall I use the diluent 2x/4x DPBS or mili Q water?

09 October 2016 3,333 4 View

What osmolality can sperm handle ?

I am trying to remove the electrostatically bound proteins using DPBS at various concentrations viz. 2X, 4X, 6X and 8x. The SDS-PAGE shows similar profile for 2X & 4X DPBS. I am unable to...

08 September 2016 4,465 3 View

What could be the reason for high Ct values of reference genes and GOI?

I am working on expression levels of beta defensins in epididymis. The RNA is fine as far as A260/280 or A260/230 is concerened. I fixed the RNA conc. at 2000ng while going for cDNA conversion....

07 August 2016 4,348 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Francisco Solano

Some of these proteins are glycoproteins, such as lectins, and it is possible that you need something else to release them.

Mike Hongma

If it is really electrostatic ineraction (see answer above) you can disrupt it by high neutral solt concentration (NaCl, KNO3 etc, conc from about 0,5M to saturation)