How can this graph be plotted for analysis of gene expression pattern?

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Why the area measure is ZERO while using multi-point tool in ImageJ?

I am trying to count the number of sperm cells in a fluorescent labelled image. After using the multi-point tool when I click measure the area column for all the selected cells is ZERO. I need...

11 December 2018 8,392 3 View

What should be the sample in selection pressure analysis ?

While performing Phylogenetic and evolutionary analysis of a gene family what should be the sample ? I am working on a gene family that has undergone various duplication & speciation events...

08 September 2018 6,859 3 View

What is the difference between 2(-ddct) and fold change and relative expression?

I have calculated 2 (-ddct) for a set of 6 genes in 5 tissues using GAPDH as reference and one of the tissues as the calibrator. However, after computing the 2 (-ddct) values, which I believe is...

07 August 2018 1,622 1 View

How can I choose an appropriate Housekeeper if there is a huge intra sample variation in Ct values?

If there is a large intra sample variation amongst the housekeeping genes, how can I decide the best housekeeper. The samples are from a abattoir which show large variations in Cq values in the...

05 June 2018 7,390 4 View

Why do I get a large number of proteins post PI-PLC treatment of sperm?

I have treated 100 million spermatozoa with PI-PLC (2U/ml) in sp-TALP medium.The enzyme has specificity for GPI anchor. However, post MS analysis by TPP, approx 300 proteins are identified many...

02 March 2018 8,952 1 View

RNA integrity ?

Does any of the RNAs look good? I want to proceed for RACE. It is a 1.2% TAE gel. Not a denaturing gel. lane 1 is ds DNA ladder (1 Kb). Although I agree it should have been run for some more time....

05 June 2017 6,611 4 View

What will be the blank in bradford's assay?

I have extracted surface proteins from a sample in 2x and 4x DPBS. While preparing standards (BSA) for quantification shall I use the diluent 2x/4x DPBS or mili Q water?

09 October 2016 3,333 4 View

Surface extraction of proteins

I am trying to remove electrostatically bound proteins on sperm surface. Is treating the sperm with 2x/4x DPBS a feasible option since I believe it would disrupt the electrostatic in PPIs.?

09 October 2016 5,648 2 View

What osmolality can sperm handle ?

I am trying to remove the electrostatically bound proteins using DPBS at various concentrations viz. 2X, 4X, 6X and 8x. The SDS-PAGE shows similar profile for 2X & 4X DPBS. I am unable to...

08 September 2016 4,465 3 View

What could be the reason for high Ct values of reference genes and GOI?

I am working on expression levels of beta defensins in epididymis. The RNA is fine as far as A260/280 or A260/230 is concerened. I fixed the RNA conc. at 2000ng while going for cDNA conversion....

07 August 2016 4,348 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View