How can this graph be plotted for analysis of gene expression pattern?

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Why the area measure is ZERO while using multi-point tool in ImageJ?

I am trying to count the number of sperm cells in a fluorescent labelled image. After using the multi-point tool when I click measure the area column for all the selected cells is ZERO. I need...

11 December 2018 8,595 3 View

What should be the sample in selection pressure analysis ?

While performing Phylogenetic and evolutionary analysis of a gene family what should be the sample ? I am working on a gene family that has undergone various duplication & speciation events...

08 September 2018 7,033 3 View

What is the difference between 2(-ddct) and fold change and relative expression?

I have calculated 2 (-ddct) for a set of 6 genes in 5 tissues using GAPDH as reference and one of the tissues as the calibrator. However, after computing the 2 (-ddct) values, which I believe is...

07 August 2018 1,836 1 View

How can I choose an appropriate Housekeeper if there is a huge intra sample variation in Ct values?

If there is a large intra sample variation amongst the housekeeping genes, how can I decide the best housekeeper. The samples are from a abattoir which show large variations in Cq values in the...

05 June 2018 7,536 4 View

Why do I get a large number of proteins post PI-PLC treatment of sperm?

I have treated 100 million spermatozoa with PI-PLC (2U/ml) in sp-TALP medium.The enzyme has specificity for GPI anchor. However, post MS analysis by TPP, approx 300 proteins are identified many...

02 March 2018 9,083 1 View

RNA integrity ?

Does any of the RNAs look good? I want to proceed for RACE. It is a 1.2% TAE gel. Not a denaturing gel. lane 1 is ds DNA ladder (1 Kb). Although I agree it should have been run for some more time....

05 June 2017 6,766 4 View

What will be the blank in bradford's assay?

I have extracted surface proteins from a sample in 2x and 4x DPBS. While preparing standards (BSA) for quantification shall I use the diluent 2x/4x DPBS or mili Q water?

09 October 2016 3,504 4 View

Surface extraction of proteins

I am trying to remove electrostatically bound proteins on sperm surface. Is treating the sperm with 2x/4x DPBS a feasible option since I believe it would disrupt the electrostatic in PPIs.?

09 October 2016 5,823 2 View

What osmolality can sperm handle ?

I am trying to remove the electrostatically bound proteins using DPBS at various concentrations viz. 2X, 4X, 6X and 8x. The SDS-PAGE shows similar profile for 2X & 4X DPBS. I am unable to...

08 September 2016 4,613 3 View

What could be the reason for high Ct values of reference genes and GOI?

I am working on expression levels of beta defensins in epididymis. The RNA is fine as far as A260/280 or A260/230 is concerened. I fixed the RNA conc. at 2000ng while going for cDNA conversion....

07 August 2016 4,484 4 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View