11 Questions 38 Answers 0 Followers
Questions related from Vipul Batra
I am trying to count the number of sperm cells in a fluorescent labelled image. After using the multi-point tool when I click measure the area column for all the selected cells is ZERO. I need...
12 December 2018 8,525 3 View
While performing Phylogenetic and evolutionary analysis of a gene family what should be the sample ? I am working on a gene family that has undergone various duplication & speciation events...
09 September 2018 6,944 3 View
I have calculated 2 (-ddct) for a set of 6 genes in 5 tissues using GAPDH as reference and one of the tissues as the calibrator. However, after computing the 2 (-ddct) values, which I believe is...
08 August 2018 1,759 1 View
If there is a large intra sample variation amongst the housekeeping genes, how can I decide the best housekeeper. The samples are from a abattoir which show large variations in Cq values in the...
06 June 2018 7,472 4 View
I have treated 100 million spermatozoa with PI-PLC (2U/ml) in sp-TALP medium.The enzyme has specificity for GPI anchor. However, post MS analysis by TPP, approx 300 proteins are identified many...
03 March 2018 9,030 1 View
Does any of the RNAs look good? I want to proceed for RACE. It is a 1.2% TAE gel. Not a denaturing gel. lane 1 is ds DNA ladder (1 Kb). Although I agree it should have been run for some more time....
06 June 2017 6,706 4 View
I want to plot the results of my qPCR in a way similar to shown in the picture since there is lot of inherent biological variation. Could anyone please let me know which tool/program was used to...
05 May 2017 5,387 3 View
I have extracted surface proteins from a sample in 2x and 4x DPBS. While preparing standards (BSA) for quantification shall I use the diluent 2x/4x DPBS or mili Q water?
10 October 2016 3,429 4 View
I am trying to remove electrostatically bound proteins on sperm surface. Is treating the sperm with 2x/4x DPBS a feasible option since I believe it would disrupt the electrostatic in PPIs.?
10 October 2016 5,743 2 View
I am trying to remove the electrostatically bound proteins using DPBS at various concentrations viz. 2X, 4X, 6X and 8x. The SDS-PAGE shows similar profile for 2X & 4X DPBS. I am unable to...
09 September 2016 4,550 3 View
I am working on expression levels of beta defensins in epididymis. The RNA is fine as far as A260/280 or A260/230 is concerened. I fixed the RNA conc. at 2000ng while going for cDNA conversion....
08 August 2016 4,422 4 View
