Which chemical will be the best choice for inducing ROS stress? Cobalt Chloride, H2O2 and monosodium iodoacetate (MIA) ?

I wish to induce stress conditions to Adipose derived mesenchymal stem cells and for that i have these three available chemicals. Your expertise are needed.

07 August 2018 5,132 10 View

Anyone has "Human Osteoblasts-Osteoarthritis: HOb-OA" cell line in Pakistan ?

I wish to check antiinflamatory activity of plants on this cell line.

05 June 2018 5,338 0 View

What is the best total plant extracts isolation protocol?

07 August 2017 1,225 4 View

What if my GAPDH expression is different in Osteoarthritis and Normal model?

03 April 2017 6,285 2 View

Can anyone please share article on different types of scaffolds preparation?

09 October 2016 4,733 0 View

What's the better way to preserve DNA and RNA. Water or Buffer and Why?

Usually i preserve it/elute it in water but i need to know whether buffer is good than water or not?

08 September 2016 3,130 23 View

What is your opinion about genetically modified crops?

02 March 2016 5,594 4 View

Should we focus on transgenic crops production? Is cisgenesis or intragenesis approach good enough to be replaced instead of transgenesis?

I need answer as i have to present a topic on recent advances of Genetic engineering for the production of fruit/crops (bio-safety prospective).

01 February 2016 6,795 3 View

Which genome editing technique is the best, efficient and reliable among ZFN,TALEN and CRISPR CAS9?

I wish to know this for the feasibility and reliability as well as accuracy.

31 December 2015 2,412 3 View

How will we explain 1 ward distance in hierarchical cluster analysis?

It means if two ethnic groups have one ward difference then what will be our explanation for them.

05 June 2013 179 0 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View