Which chemical will be the best choice for inducing ROS stress? Cobalt Chloride, H2O2 and monosodium iodoacetate (MIA) ?

I wish to induce stress conditions to Adipose derived mesenchymal stem cells and for that i have these three available chemicals. Your expertise are needed.

07 August 2018 5,132 10 View

Anyone has "Human Osteoblasts-Osteoarthritis: HOb-OA" cell line in Pakistan ?

I wish to check antiinflamatory activity of plants on this cell line.

05 June 2018 5,338 0 View

What is the best total plant extracts isolation protocol?

07 August 2017 1,225 4 View

What if my GAPDH expression is different in Osteoarthritis and Normal model?

03 April 2017 6,285 2 View

Can anyone please share article on different types of scaffolds preparation?

09 October 2016 4,733 0 View

What's the better way to preserve DNA and RNA. Water or Buffer and Why?

Usually i preserve it/elute it in water but i need to know whether buffer is good than water or not?

08 September 2016 3,130 23 View

Should we focus on transgenic crops production? Is cisgenesis or intragenesis approach good enough to be replaced instead of transgenesis?

I need answer as i have to present a topic on recent advances of Genetic engineering for the production of fruit/crops (bio-safety prospective).

01 February 2016 6,795 3 View

Which genome editing technique is the best, efficient and reliable among ZFN,TALEN and CRISPR CAS9?

I wish to know this for the feasibility and reliability as well as accuracy.

31 December 2015 2,412 3 View

What will be the best Acid to change the PH of a buffer up to 8.0. Either i should use Strong acid or weak acid and why???

Just wish to know if there is any effect and if there is any, so how?

31 December 2015 8,637 0 View

How will we explain 1 ward distance in hierarchical cluster analysis?

It means if two ethnic groups have one ward difference then what will be our explanation for them.

05 June 2013 179 0 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View