Which chemical will be the best choice for inducing ROS stress? Cobalt Chloride, H2O2 and monosodium iodoacetate (MIA) ?

I wish to induce stress conditions to Adipose derived mesenchymal stem cells and for that i have these three available chemicals. Your expertise are needed.

07 August 2018 5,132 10 View

Anyone has "Human Osteoblasts-Osteoarthritis: HOb-OA" cell line in Pakistan ?

I wish to check antiinflamatory activity of plants on this cell line.

05 June 2018 5,338 0 View

What is the best total plant extracts isolation protocol?

07 August 2017 1,225 4 View

What if my GAPDH expression is different in Osteoarthritis and Normal model?

03 April 2017 6,285 2 View

What's the better way to preserve DNA and RNA. Water or Buffer and Why?

Usually i preserve it/elute it in water but i need to know whether buffer is good than water or not?

08 September 2016 3,130 23 View

What is your opinion about genetically modified crops?

02 March 2016 5,594 4 View

Should we focus on transgenic crops production? Is cisgenesis or intragenesis approach good enough to be replaced instead of transgenesis?

I need answer as i have to present a topic on recent advances of Genetic engineering for the production of fruit/crops (bio-safety prospective).

01 February 2016 6,795 3 View

Which genome editing technique is the best, efficient and reliable among ZFN,TALEN and CRISPR CAS9?

I wish to know this for the feasibility and reliability as well as accuracy.

31 December 2015 2,412 3 View

What will be the best Acid to change the PH of a buffer up to 8.0. Either i should use Strong acid or weak acid and why???

Just wish to know if there is any effect and if there is any, so how?

31 December 2015 8,637 0 View

How will we explain 1 ward distance in hierarchical cluster analysis?

It means if two ethnic groups have one ward difference then what will be our explanation for them.

05 June 2013 179 0 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Can't recognize exact phase and PDF card no of Silicon carbide in XRD?

We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...

22 February 2021 2,072 3 View

How can I add a new WSN routing protocol in NS2 or NS3?

I'm trying to find in literature a way to help me adding a modified Yen's algorithm in ns2 or its variance ns3 or another simulator. Have you some tips which can be helpful, I'm opening in the...

19 February 2021 7,981 3 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

How to do mono formylation of N,N-diethyl-3-methoxybenzamide?

I have attempted the formylation of the above mentioned compound using a very well reported protocol using secondary Butyl Lithium and TMEDA at - 78 degrees Celsius. I have done it quite a few...

08 February 2021 2,793 2 View

I want to simulate a privacy-preserving algorithm in MQTT for IoT devices, but I'm not sure which simulation software is best suited for this?

I want to apply a privacy-preserving algorithm in MQTT for IoT devices. Which tool do you suggest to simulate this algorithm? I want to measure the performance on the IoT battery device? Actually,...

28 January 2021 9,561 3 View

How can we simulate vehicle to infrastructure communication in NS-3?

I am working on handoff management in IEEE 802.11p/WAVE based VANETs. I want to know if I can simulate V2I communication mode using NS-3, which will lead to the simulation of handoff process. Most...

16 January 2021 1,733 4 View

How to create a scaffold by using the particle leaching process?

In the leaching process,Why does NaCl settle despite sieving? How can we understand how much solvent the system needs? And, how can enhance the mechanical strength in this process and find the...

12 January 2021 5,679 2 View

Which antimicrobial test will be suitable to determine antimicrobial activity of silver nanoparticles?

There are some journal articles that reported well diffusion method to determine the antimicrobial activity of silver nanoparticles. Meanwhile, some reported disc diffusion method. Which method is...

06 January 2021 7,123 4 View