Anyone has "Human Osteoblasts-Osteoarthritis: HOb-OA" cell line in Pakistan ?

I wish to check antiinflamatory activity of plants on this cell line.

05 June 2018 5,338 0 View

What is the best total plant extracts isolation protocol?

07 August 2017 1,225 4 View

What if my GAPDH expression is different in Osteoarthritis and Normal model?

03 April 2017 6,285 2 View

Can anyone please share article on different types of scaffolds preparation?

09 October 2016 4,733 0 View

What's the better way to preserve DNA and RNA. Water or Buffer and Why?

Usually i preserve it/elute it in water but i need to know whether buffer is good than water or not?

08 September 2016 3,130 23 View

What is your opinion about genetically modified crops?

02 March 2016 5,594 4 View

Should we focus on transgenic crops production? Is cisgenesis or intragenesis approach good enough to be replaced instead of transgenesis?

I need answer as i have to present a topic on recent advances of Genetic engineering for the production of fruit/crops (bio-safety prospective).

01 February 2016 6,795 3 View

Which genome editing technique is the best, efficient and reliable among ZFN,TALEN and CRISPR CAS9?

I wish to know this for the feasibility and reliability as well as accuracy.

31 December 2015 2,412 3 View

What will be the best Acid to change the PH of a buffer up to 8.0. Either i should use Strong acid or weak acid and why???

Just wish to know if there is any effect and if there is any, so how?

31 December 2015 8,637 0 View

How will we explain 1 ward distance in hierarchical cluster analysis?

It means if two ethnic groups have one ward difference then what will be our explanation for them.

05 June 2013 179 0 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

No title

02 March 2021 3,060 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View