Which chemical will be the best choice for inducing ROS stress? Cobalt Chloride, H2O2 and monosodium iodoacetate (MIA) ?

I wish to induce stress conditions to Adipose derived mesenchymal stem cells and for that i have these three available chemicals. Your expertise are needed.

07 August 2018 5,132 10 View

Anyone has "Human Osteoblasts-Osteoarthritis: HOb-OA" cell line in Pakistan ?

I wish to check antiinflamatory activity of plants on this cell line.

05 June 2018 5,338 0 View

What if my GAPDH expression is different in Osteoarthritis and Normal model?

03 April 2017 6,285 2 View

Can anyone please share article on different types of scaffolds preparation?

09 October 2016 4,733 0 View

What's the better way to preserve DNA and RNA. Water or Buffer and Why?

Usually i preserve it/elute it in water but i need to know whether buffer is good than water or not?

08 September 2016 3,130 23 View

What is your opinion about genetically modified crops?

02 March 2016 5,594 4 View

Should we focus on transgenic crops production? Is cisgenesis or intragenesis approach good enough to be replaced instead of transgenesis?

I need answer as i have to present a topic on recent advances of Genetic engineering for the production of fruit/crops (bio-safety prospective).

01 February 2016 6,795 3 View

Which genome editing technique is the best, efficient and reliable among ZFN,TALEN and CRISPR CAS9?

I wish to know this for the feasibility and reliability as well as accuracy.

31 December 2015 2,412 3 View

What will be the best Acid to change the PH of a buffer up to 8.0. Either i should use Strong acid or weak acid and why???

Just wish to know if there is any effect and if there is any, so how?

31 December 2015 8,637 0 View

How will we explain 1 ward distance in hierarchical cluster analysis?

It means if two ethnic groups have one ward difference then what will be our explanation for them.

05 June 2013 179 0 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View