I have a question on cultured cell staying in RPMI medium for a month without an idea what is it.
Some informations are;
Source of sample: blood sample from cancer patient.
Separation method: electrical field.
Cell culture: Isolated cells have been cultured with RPMI1640 + 20%FBS + antibiotics.
My guess is that blood cells should not attach to Well Plate and can be skimmed off. The attached cell could be epithelial or endothelial cells?