I have a question on cultured cell staying in RPMI medium for a month without an idea what is it.

Some informations are;

Source of sample: blood sample from cancer patient.

Separation method: electrical field.

Cell culture: Isolated cells have been cultured with RPMI1640 + 20%FBS + antibiotics.

My guess is that blood cells should not attach to Well Plate and can be skimmed off. The attached cell could be epithelial or endothelial cells?

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