Deepak N Modi

3 Questions 37 Answers 0 Followers

Questions related from Deepak N Modi

Is there a restriction in Amplicon size for ddPCR ?

Hello , We need to do ddPCR (Eva green chemistry) for a primer pair that yields a 429 bp amplicon. It is a single band on gel. I understand that there are reason for restriction amplicon size in...

04 April 2019 4,053 3 View

Does any one have a good protocol for in vivo transfections in mouse uterus and ex vivo in intact tissues?

I am trying to transfect a plasmid in vivo in the decidua of E5.5-6.5 pregnant mouse uterus and also ex vivo in whole organ cultures. I do not want to use electroporation or viral transduction. I...

07 July 2013 7,006 2 View

In RNAseq biological or technical replicates are prefered ?

I am working on a very limited budget RNAseq. We have 4-5 human biopsy samples from three groups (one control an two test) . I wish to know if 1) we should pool the RNA from biological replicates...

01 January 1970 4,609 8 View