Mohamed Kamal

7 Questions 27 Answers 0 Followers

Questions related from Mohamed Kamal

What kit or method is suitable for the extraction of RNA from around 50 cells?

Dear All, I am wondering if you could recommend a good kit or method for the extraction of RNA from as low as 50 cells. I need to run RNA seq afterwords. Looking forward to your...

01 January 2018 1,482 2 View

Could a tumour suppressor gene enhance the activation of PI3K pathways?

I work on a tumour suppressor gene in breast cancer. The down regulation of this gene in MCF10A cells (using shRNA) caused a 12 folds decrease in the mRNA expression of PI3K catalytic subunit...

01 January 2014 981 8 View

Do I have to confirm the qPCR array data using specific qPCR assays?

I ran qPCR arrays to test the expression of 48 genes in two samples. 10 genes showed significant difference between samples. The assay was very reproducible (nearly same results between...

10 October 2013 3,791 8 View

Do I have to confirm the qPCR array (Stell Array, Lonza) data using specific qPCR assays before testing the protein expression of these genes?

I have used qPCR arrays to test the expression of 48b genes in two groups of cells. These arrays showed 10 genes which their expressions are significantly different between the groups. The next...

09 September 2013 7,160 2 View

Is there any way to ship cell lysates (for western and Co-IP) abroad?

The next step of my project is to do western blotting and Co-IP. However, the cells which I will use are not in my lab, they are in my collaborator lab abroad. Because I do not have cell culture...

12 December 2012 4,804 2 View

What are the best ways for selecting genes to be included in qPCR arrays?

I am currently designing qPCR array to investigate the effect of my gene of interest (CSMD1) on the expression of a number of genes. My cell culture studies showed that CSMD1 affects cell shape,...

05 May 2012 2,009 7 View

Does anyone tried mammosphere assay for circulating tumour cells?

I am wondering if it is possible to do mammosphere assay for individual Circulating tumour cells. If someone has experience in this it would be much appreciated to share it with me. Thanks

01 January 1970 5,364 3 View