ANCOVA controlling baseline in RCT - how to perform power analysis and sample size estimation?

Hi, I have a simple question. I am hoping to perform a power analysis/sample size estimation for an RCT. We will be controlling for baseline symptoms, and using post-treatment or change scores as...

19 February 2021 2,648 23 View

Why am I getting western bands with white bubbles?

12 October 2020 6,976 3 View

How would I determine if a protein (PD-1) is necessary for establishment of latent infection by a virus in a CD4 memory cell line?

If I were to plan an experiment to deduce whether or not PD-1 is absolutely necessary for HIV to establish latent infection in CD4 memory cells, how would I go about doing this? More...

09 March 2020 7,774 2 View

What is the most appropriate statistical analysis to assess between and within subject differences over time with most individuals with 2 time points?

I am looking at how hormone concentrations change over time both between individuals and in relation to fixed effects of predictor variables (n=7) within individuals. I have a large dataset of...

02 July 2019 8,734 9 View

I want to selecte resistance antibiotic bateria in the pond ? I was offer the MHA already but Could you give me some idea about an other medium ?

The antibiotic target is SMX and OCT,

30 June 2019 5,542 5 View

Calculating beam nodal displacements/rotations using SAP2000 and programming system matrices in MATLAB - Differences in responses?

I have programmed an Euler-Bernoulli beam, supported on springs, using both SAP2000, and by using the stiffness matrix method in MATLAB. The nodal (joint) displacements calculated due to an...

13 March 2019 8,597 3 View

Caco-2 cells showing high TEER (2000ohm cm2) after 21 days growth, is this normal?

I am working on the Caco-2 cell line, I'm particularly interested in changes in permeability under inflammatory stimulation. I am using growth conditions: 12-well transwell plates, seeding...

14 January 2019 3,047 2 View

Why Aren't My 1L Bacterial Cultures Growing Properly?

I performed a fresh transformation (with sequenced plasmid) into commercial BL21 competent cells. I scrape a colony into a starter culture w/ antibiotic overnight. I check the O.D. of the starter...

15 October 2018 3,171 9 View

How does emulsion polymerization work?

I would like a simple explanation of emulsion polymerization, and I would like to know whether the process requires UV light and how UV light will effect the process.

15 March 2018 7,706 3 View

Does Abcam's anti-TrkB antibody (ab18987) also bind activated or phosphorylated TrkB (p-TrkB)?

I've been using this anti-TrkB antibody in studies involving the activation of TrkB by BDNF. When I called Abcam to ask if it also binds p-TrkB, I was told that they've never tested it, and have...

20 February 2018 2,758 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View