I am asking this as all questions that I can find concern more basic and widespread detachment problems but ours is recent and seems to occur at a specific passage.
We have multiple iPS cell lines that we reprogrammed and some that we purchased. They were behaving as expected until recently. Now we have an interesting problem. Let's say we take a p23-24 line. We thaw and plate on matrigel in mTESR/mTESR+ or E8 (rok1-i
IPSC (induced pluripotent stem cell) cell detachment means that these cells are no longer firmly attached to the surface of the medium during culture, but detach. This can affect cell growth and viability, reducing the efficiency and cost of cell culture.
In terms of the cause of IPSC cell detachment, there may be several factors as follows:
1. Improper cell culture conditions: The culture environment of cells includes factors such as culture medium, temperature, and pH value. Unsuitable conditions may cause cells to detach.
2. Problems with the culture surface: Poor quality of the coating on the culture surface or the cell culture vessel, or improper coating materials, etc., may cause the cells to detach.
3. Improper cell density: too high or too low cell density may lead to cell detachment.
Solutions for IPSC cell detachment can consider the following aspects:
1. Optimize culture conditions: adjust the composition, temperature and pH value of the cell culture medium to provide a suitable growth environment and increase the ability of cell attachment.
2. Improve the culture surface: optimize the coating material of the cell culture vessel to ensure that the cells can be better attached to the surface.
3. Control cell density: According to different cell types and needs, control the appropriate cell density to avoid cell detachment caused by too high or too low.
4. Use cell adhesives: Some cell adhesives can increase the ability of cells to adhere to the culture surface, and these adhesives can be considered to increase cell attachment.
5. Improvement of culture technology: New culture technology, such as three-dimensional culture or scaffold culture, can provide a better environment for cell attachment and reduce the problem of cell detachment.
In summary, the solution for IPSC cell detachment needs to comprehensively consider factors such as culture conditions, culture surface and cell density, and optimize and improve according to the specific situation.
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