Frances Marin

3 Questions 5 Answers 0 Followers

Questions related from Frances Marin

Which additive would be appropriate when collecting peripheral blood to isolate plasma and PBMC?

We expect collect 10 ml of peripheral blood in order to isolate plasma and PBMC , after a density gradient separation with Lymphoprep using sep mate tubes. The collected plasma and PBMC will be...

23 May 2019 4,471 3 View

Anyone has a protocol to culture eosinophils isolated from human peripheral blood?

With the recommended medium and supplements, time for subculturing, etc?

29 January 2018 9,628 7 View

Fluo-4 Direct Calcium Assay Kit and the use of EDTA in peripheral blood collection?

I will be isolating eosinophils from peripheral blood and after that I will use the cells to evaluate intracellular levels of Ca using Fluo-4 Direct Calcium Assay Kit (Invitrogen). I would like to...

24 January 2018 4,804 2 View